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http://purl.uniprot.org/citations/32182608http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objective

MiR-203 has been shown to participate in multiple malignancies, but the role of miR-203 in hepatoblastoma (HB) remains unclear. The aim of our study was to investigate the effects of miR-203 in HB.

Methods

A total of 15 pairs of HB tissues and para-tumour normal tissues were collected for the experiments. RT-qPCR and Western blotting were performed to detect the expression of CRNDE, miR-203, and VEGFA at the mRNA and/or protein levels, respectively. A dual luciferase assay verified the target relationship between miR-203 and the 3'UTR of VEGFA as well as miR-203 and CRNDE. In addition, MTT, wound healing, and tube formation assays were performed to assess the effects of miR-203, VEGFA, and CRNDE on cell proliferation, migration, and angiogenesis, respectively.

Results

Our data revealed that miR-203 expression was decreased in HB tissues, while long non-coding RNA (lncRNA) CRNDE expression was increased. The dysregulation of miR-203 and CRNDE was closely related to tumour size and stage. Moreover, overexpression of miR-203 inhibited angiogenesis. A dual luciferase assay verified that VEGFA is a direct target of miR-203 and that CRNDE binds to miR-203. Furthermore, our results showed that miR-203 suppressed cell viability, migration, and angiogenesis by regulating VEGFA expression. Additionally, it was confirmed that CRNDE promoted angiogenesis by negatively regulating miR-203 expression.

Conclusion

lncRNA CRNDE targets the miR-203/VEGFA axis and promotes angiogenesis in HB. These results provide insight into the underlying mechanisms of HB and indicate that CRNDE and miR-203 might be potential targets for HB therapy."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.org/dc/terms/identifier"doi:10.1159/000505131"xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Zhang T."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Zhang Y.B."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Yin Q."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Peng Y.M."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Chen L.J."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Liu Z.Y."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Ji C.Y."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Sheng X.Y."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Yuan M.X."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Gao H.Q."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/author"Xie W.X."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/name"Pathobiology"xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/pages"161-170"xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/title"Long Non-Coding RNA CRNDE Regulates Angiogenesis in Hepatoblastoma by Targeting the MiR-203/VEGFA Axis."xsd:string
http://purl.uniprot.org/citations/32182608http://purl.uniprot.org/core/volume"87"xsd:string
http://purl.uniprot.org/citations/32182608http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32182608
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http://purl.uniprot.org/uniprot/#_A2A2V4-mappedCitation-32182608http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/32182608
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