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http://purl.uniprot.org/citations/32201961http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32201961http://www.w3.org/2000/01/rdf-schema#comment"

Background and objectives

The Mi(a+) GP(B-A-B) hybrid phenotypes occur with a prevalence of 2%-23% across Southeast Asia. While the s antigen is alleged to be altered, no evidence for specific variants is known. Screening using a monoclonal IgM anti-s mistyped six S-s+ RBC units as S-s-. Further, alloanti-s was identified in an S+s+ patient. Our objective was to investigate the s antigen further.

Materials and methods

DNA from 63 Thai blood donor samples PCR-positive for a GYP(B-A-B) hybrid was sequenced with primers spanning GYPB exons 3-4. Flow cytometry was used for semiquantitative analysis of s expression and correlated with the glycophorin genotype.

Results

DNA sequencing showed that GYP*Mur was carried by 56/63 samples (88·9%) of which 5/56 lacked normal GYPB: three of these were GYP*Mur homozygotes, one was a compound heterozygote carrying GYP*Mur and a GYP*Bun-like allele (designated GYP*Thai), and the fifth sample carried GYP*Mur and another GYP*Bun-like allele. Seven samples (7/63) were GYP*Thai heterozygotes. IgM monoclonal anti-s (P3BER) did not react with the s antigen carried by GP.Mur or GP.Bun, whereas two IgG anti-s showed enhanced reactivity.

Conclusions

We confirmed that GYP*Mur is the most frequent variant in Thai blood donors and also identified GYP*Thai with a frequency of 1·1%. We showed that s antigen on Mi(a+) GP(B-A-B) hybrids is qualitatively altered and should be considered when selecting reagents for phenotyping where such hybrids are prevalent, endemically and in blood centres worldwide."xsd:string
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http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/author"Olsson M.L."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/author"Storry J.R."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/author"Thornton N."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/author"Grimsley S."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/author"Robb J."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/author"Jongruamklang P."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/name"Vox Sang"xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/pages"472-477"xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/title"Characterization of GYP*Mur and novel GYP*Bun-like hybrids in Thai blood donors reveals a qualitatively altered s antigen."xsd:string
http://purl.uniprot.org/citations/32201961http://purl.uniprot.org/core/volume"115"xsd:string
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