| http://purl.uniprot.org/citations/32203490 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32203490 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32203490 | http://www.w3.org/2000/01/rdf-schema#comment | "Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by degradation of nascent peptides. However, endogenous RQC-inducing sequences and the mechanism underlying the ubiquitin-dependent ribosome dissociation remain poorly understood. Here, we identified SDD1 messenger RNA from Saccharomyces cerevisiae as an endogenous RQC substrate and reveal the mechanism of its mRNA-dependent and nascent peptide-dependent translational stalling. In vitro translation of SDD1 mRNA enabled the reconstitution of Hel2-dependent polyubiquitination of collided disomes and, preferentially, trisomes. The distinct trisome architecture, visualized using cryo-EM, provides the structural basis for the more-efficient recognition by Hel2 compared with that of disomes. Subsequently, the Slh1 helicase subunit of the RQC trigger (RQT) complex preferentially dissociates the first stalled polyubiquitinated ribosome in an ATP-dependent manner. Together, these findings provide fundamental mechanistic insights into RQC and its physiological role in maintaining cellular protein homeostasis."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41594-020-0393-9"xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41594-020-0393-9"xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Cheng J."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Cheng J."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Inada T."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Inada T."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Mizuno M."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Mizuno M."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Matsuo Y."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Matsuo Y."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Buschauer R."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Buschauer R."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Endo A."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Endo A."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Becker T."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Becker T."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Beckmann R."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Beckmann R."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Nakajima S."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Nakajima S."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Ikeuchi K."xsd:string |
| http://purl.uniprot.org/citations/32203490 | http://purl.uniprot.org/core/author | "Ikeuchi K."xsd:string |