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http://purl.uniprot.org/citations/32298762http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32298762http://www.w3.org/2000/01/rdf-schema#comment"

Objective

We analysed the effect of expression of nucleolar spindle-associated protein 1 (NuSAP1) on the prognosis of breast cancer (BC) and investigated its potential mechanism of tumourigenicity in triple-negative breast cancer (TNBC) cell lines.

Materials and methods

We downloaded the RNA-seq breast cancer (BC) data from The Cancer Genome Atlas (TCGA) and screened for the NuSAP1 gene using R software. The clinical data for patients with BC were screened and analysed using R software. A survival curve was drawn using the Kaplan-Meier Plotter. Cell proliferation and invasion were verified by the Cell Counting Kit-8 and Transwell assays. Expression of NuSAP1, the Wnt/β-catenin pathway, and epithelial-mesenchymal-transition-related proteins in TNBC was detected using real-time quantitative polymerase chain reaction (qRT-PCR) and western blotting (WB).

Results

Expression of NuSAP1 was upregulated in BC. The change in NuSAP1 expression levels was associated with multiple clinicopathological factors, and the higher the expression of NuSAP1 was, the shorter the survival time. In MDA-MB-231 and BT549 cells, knockdown of NuSAP1 expression resulted in a significant decrease in cell proliferation and invasion; a decrease in expression of cyclin D1, vimentin, Slug, Twist, wnt3a, and pβ-catenin; and an increase in expression of e-cadherin. The results of the sh-NuSAP1 + ov-NuSPA1 group were the opposite of the results of the sh-NuSAP1 group.

Conclusion

NuSAP1 is a carcinogen that facilitates progression of TNBC through the Wnt/β-catenin and epithelial-mesenchymal transition pathways."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.org/dc/terms/identifier"doi:10.1016/j.gene.2020.144657"xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Liu S."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Sun L."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Shi C."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Yan L."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Zhao Y."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Ji C."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/author"Zhang E."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/pages"144657"xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/title"Overexpression of NuSAP1 is predictive of an unfavourable prognosis and promotes proliferation and invasion of triple-negative breast cancer cells via the Wnt/beta-catenin/EMT signalling axis."xsd:string
http://purl.uniprot.org/citations/32298762http://purl.uniprot.org/core/volume"747"xsd:string
http://purl.uniprot.org/citations/32298762http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32298762
http://purl.uniprot.org/citations/32298762http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/32298762
http://purl.uniprot.org/uniprot/#_A8K4B4-mappedCitation-32298762http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/32298762
http://purl.uniprot.org/uniprot/#_Q9BXS6-mappedCitation-32298762http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/32298762
http://purl.uniprot.org/uniprot/A8K4B4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/32298762
http://purl.uniprot.org/uniprot/Q9BXS6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/32298762