http://purl.uniprot.org/citations/32325255 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/32325255 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/32325255 | http://www.w3.org/2000/01/rdf-schema#comment | "The endo-β-1,4-mannanase from the hyperthermostable bacterium Thermotoga petrophila (TpMan) is an enzyme that catalyzes the hydrolysis of mannan and heteromannan polysaccharides. Of the three domains that comprise TpMan, the N-terminal GH5 catalytic domain and the C-terminal carbohydrate-binding domain are connected through a central ancillary domain of unknown structure and function. In this study, we report the partial crystal structure of the TpMan at 1.45 Å resolution, so far, the first modular hyperthermostable endo-β-1,4-mannanase structure determined. The structure exhibits two domains, a (β/α)8-barrel GH5 catalytic domain connected via a linker to the central domain with an immunoglobulin-like β-sandwich fold formed of seven β-strands. Functional analysis showed that whereas the immunoglobulin-like domain does not have the carbohydrate-binding function, it stacks on the GH5 catalytic domain acting as a thermostabilizing domain and allowing operation at hyperthermophilic conditions. The carbohydrate-binding domain is absent in the crystal structure most likely due to its high flexibility around the immunoglobulin-like domain which may act also as a pivot. These results represent new structural and functional information useful on biotechnological applications for biofuel and food industries."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbapap.2020.140437"xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbapap.2020.140437"xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Nicolet Y."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Nicolet Y."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Martin L."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Martin L."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Squina F.M."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Squina F.M."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Speranca M.A."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Speranca M.A."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Garcia W."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Garcia W."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Muniz J.R.C."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Muniz J.R.C."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Cabral A.D."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "Cabral A.D."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "da Silva V.M."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/author | "da Silva V.M."xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Proteins Proteom"xsd:string |
http://purl.uniprot.org/citations/32325255 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Proteins Proteom"xsd:string |