| http://purl.uniprot.org/citations/32327568 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32327568 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32327568 | http://www.w3.org/2000/01/rdf-schema#comment | "Misfolded luminal endoplasmic reticulum (ER) proteins undergo ER-associated degradation (ERAD-L): They are retrotranslocated into the cytosol, polyubiquitinated, and degraded by the proteasome. ERAD-L is mediated by the Hrd1 complex (composed of Hrd1, Hrd3, Der1, Usa1, and Yos9), but the mechanism of retrotranslocation remains mysterious. Here, we report a structure of the active Hrd1 complex, as determined by cryo-electron microscopy analysis of two subcomplexes. Hrd3 and Yos9 jointly create a luminal binding site that recognizes glycosylated substrates. Hrd1 and the rhomboid-like Der1 protein form two "half-channels" with cytosolic and luminal cavities, respectively, and lateral gates facing one another in a thinned membrane region. These structures, along with crosslinking and molecular dynamics simulation results, suggest how a polypeptide loop of an ERAD-L substrate moves through the ER membrane."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.aaz2449"xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.aaz2449"xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Liao M."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Liao M."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Mi W."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Mi W."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Nudler E."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Nudler E."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Wu X."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Wu X."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Rapoport T.A."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Rapoport T.A."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Hummer G."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Hummer G."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Ovchinnikov S."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Ovchinnikov S."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Siggel M."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Siggel M."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Svetlov V."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/author | "Svetlov V."xsd:string |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32327568 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |