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http://purl.uniprot.org/citations/32338277http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32338277http://www.w3.org/2000/01/rdf-schema#comment"

Background

microRNAs (miRs) regulate the expression of protein-coding genes and play key roles in various biological processes, including development and immunity. However, dysregulation of miR expression is also involved in disease biology, including cancer.

Methods

We utilized The Cancer Genome Atlas (TCGA) and other publicly available databases for miRs and mRNA expression in prostate cancer, selected miR-484 and investigated its role in prostate cancer biology and disease progression using in vitro studies.

Results

Our data mining efforts revealed that increased miR-484 in prostate tumors associates with early disease recurrence, while miR-484 expression in human prostate cancer cells enhances cancer cell mobility. Using RNAseq and bioinformatics, we identified candidate target genes of miR-484 and generated a list of potential tumor suppressors. One candidate in this list was PSMG1. We applied luciferase assays and immunoblotting to confirm that miR-484 directly targets PSMG1. Additional in vitro assays with cancer cell lines showed that PSMG1 knockdown rescued the reduction in mobility brought on by miR-484 inhibition, pointing toward the existence of a miR-484-PSMG1 axis in prostate cancer.

Conclusions

We hypothesize that miR-484 is an oncogene in the prostate that increases cancer cell mobility, with PSMG1 being a mir-484 target in this process."xsd:string
http://purl.uniprot.org/citations/32338277http://purl.org/dc/terms/identifier"doi:10.1042/bsr20191028"xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/author"Lee D."xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/author"Tang W."xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/author"Ambs S."xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/author"Dorsey T.H."xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/name"Biosci Rep"xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/pages"BSR20191028"xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/title"miR-484 is associated with disease recurrence and promotes migration in prostate cancer."xsd:string
http://purl.uniprot.org/citations/32338277http://purl.uniprot.org/core/volume"40"xsd:string
http://purl.uniprot.org/citations/32338277http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32338277
http://purl.uniprot.org/citations/32338277http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/32338277
http://purl.uniprot.org/uniprot/#_B2RD51-mappedCitation-32338277http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/32338277
http://purl.uniprot.org/uniprot/#_O95456-mappedCitation-32338277http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/32338277
http://purl.uniprot.org/uniprot/B2RD51http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/32338277
http://purl.uniprot.org/uniprot/O95456http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/32338277