| http://purl.uniprot.org/citations/32427100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32427100 | http://www.w3.org/2000/01/rdf-schema#comment | "Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from E. coli dnaX mRNA and the gag-pol transcript of Human Immunodeficiency Virus (HIV) perturb translation elongation. We find that upon encountering the ribosome, the stem-loops strongly inhibit A-site tRNA binding and ribosome intersubunit rotation that accompanies translation elongation. Electron cryo-microscopy (cryo-EM) reveals that the HIV stem-loop docks into the A site of the ribosome. Our results suggest that mRNA stem-loops can transiently escape the ribosome helicase by binding to the A site. Thus, the stem-loops can modulate gene expression by sterically hindering tRNA binding and inhibiting translation elongation."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.55799"xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/author | "Grigorieff N."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/author | "Ermolenko D.N."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/author | "Ling C."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/author | "Bao C."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/author | "Korostelev A.A."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/author | "Loerch S."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/pages | "e55799"xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/title | "mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding."xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/32427100 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32427100 |
| http://purl.uniprot.org/citations/32427100 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32427100 |
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