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http://purl.uniprot.org/citations/32450789http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32450789http://www.w3.org/2000/01/rdf-schema#comment"

Background

MicroRNAs (miRNAs) play crucial roles in regulating eukaryotic gene expression. Recent studies indicated that aberrantly expressed miRNAs are involved in the pathogenesis of ankylosing spondylitis (AS). Indeed, hsa-miR-495-3p (miR-495) has been reported as an anti-oncogene in different cancers. However, the role of miR-495 in AS is still unknown.

Methods

In this study, quantitative real-time polymerase chain reaction (PCR) was used to detect the expression of miR-495 in the peripheral blood mononuclear cells (PBMCs), whole blood, and serum of patients with AS. Bisulfite-specific PCR sequencing and methylated DNA immunoprecipitation were used to detect the methylation in the promoter region of miR-495. To determine the influence of miR-495 expression on the target gene, programmed cell death 10 (PDCD10), dual luciferase reporter assays together with an adenoviral vector containing the miR-495 locus were used. Receiver operating characteristic (ROC) curves were used to evaluate the efficacy of miR-495 as a diagnostic biomarker of AS. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and western blotting were used to explore the potential role of miR-495 in AS pathogenesis and the mechanism by which it facilitates AS pathogenesis.

Results

miR-495 is down-regulated and the promoter region of miR-495 is highly methylated in AS. The expression of miR-495 is negatively associated with PDCD10 expression in both patients with AS and healthy controls. Further experiments showed that PDCD10 can be targeted by miR-495. The ROC curves of miR-495 suggested that it is a very specific and sensitive biomarker for AS diagnosis. Bioinformatics analysis and signal pathway studies indicated that miR-495 can down-regulate β-catenin and transforming growth factor-β1.

Conclusions

Our studies indicated that down-regulation of miR-495 can be used as a potential molecular marker for the diagnosis and treatment of AS, thus providing new insights into the role of miRNAs in AS pathology."xsd:string
http://purl.uniprot.org/citations/32450789http://purl.org/dc/terms/identifier"doi:10.1186/s10020-020-00157-3"xsd:string
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/author"Leng X.M."xsd:string
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/author"Ni W.J."xsd:string
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/name"Mol Med"xsd:string
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/pages"50"xsd:string
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/title"Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis."xsd:string
http://purl.uniprot.org/citations/32450789http://purl.uniprot.org/core/volume"26"xsd:string
http://purl.uniprot.org/citations/32450789http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32450789
http://purl.uniprot.org/citations/32450789http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/32450789
http://purl.uniprot.org/uniprot/#_C9J363-mappedCitation-32450789http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/32450789
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http://purl.uniprot.org/uniprot/Q9BUL8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/32450789
http://purl.uniprot.org/uniprot/C9J363http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/32450789