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http://purl.uniprot.org/citations/32492224http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32492224http://www.w3.org/2000/01/rdf-schema#comment"

Background

Several cancers, including mycosis fungoides (MF), have reported dysregulation of miR-195-5p. miR-195-5p plays a role in cell cycle regulation in several malignant diseases.

Objectives

This study aimed to investigate: (a) the expression level of miR-195-5p in lesional MF skin biopsies and (b) the potential regulatory roles of miR-195-5p in MF.

Methods

Quantitative real-time polymerase chain reaction (RT-qPCR) was used to determine miR-195-5p expression in MF skin biopsies and cell lines. The effect of miR-195-5p and ADP-ribosylation factor-like protein 2 (ARL2) on cell cycle and apoptosis was measured by flow cytometry assays. Changes in ARL2 expression were determined by RT-qPCR and Western blotting (WB).

Results

We found lower expression levels of miR-195-5p in lesional skin from MF patients compared with non-lesional MF skin and skin from healthy volunteers. Additionally, miR-195-5p showed lower expression levels in the skin from patients with disease progression compared with patients with stable disease. In vitro studies showed that overexpression of miR-195-5p induced a cell cycle arrest in G0G1. Using microarray analysis, we identified several genes that were regulated after miR-195-5p overexpression. The most downregulated gene after miR-195-5p mimic transfection was ARL2. RT-qPCR and WB analyses confirmed downregulation of ARL2 following transfection with miR-195-5p mimic. Lastly, transfection with siRNA against ARL2 also induced a G0G1 arrest.

Conclusion

Upregulation of miR-195-5p in MF inhibits cycle arrest by downregulation of ARL2. miR-195-5p may thus function as a tumor suppressor in MF and low miR-195-5p expression in lesional MF skin may promote disease progression."xsd:string
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http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/author"Celis P."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/author"Johansen C."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/author"Iversen L."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/author"Woetmann A."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/author"Lindahl L.M."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/author"Rittig A.H."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/name"Exp Dermatol"xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/pages"1141-1149"xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/title"Suppressed microRNA-195-5p expression in mycosis fungoides promotes tumor cell proliferation."xsd:string
http://purl.uniprot.org/citations/32492224http://purl.uniprot.org/core/volume"30"xsd:string
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