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http://purl.uniprot.org/citations/32495858http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32495858http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Osteoarthritis (OA) is a common disease in the elderly and seriously affects the quality of life of patients. The purpose of this study was to explore the protective effect of Fibulin-5 on articular chondrocytes and its mechanism of action.

Patients and methods

Articular cartilage tissues from patients with OA and normal people were selected and tested for differences in Fibulin-5 expression. In addition, human chondrocytes were cultured, and the effects of Fibulin-5 on the extracellular matrix (ECM) of chondrocytes and the level of inflammation were examined by means of cell transfection and cytokine intervention. SKL2001, an agonist of the Wnt/β-catenin signaling pathway, was used to validate the mechanism of action of Fibulin-5 to protect chondrocytes.

Results

Fibulin-5 was lowly expressed in the cartilage tissue of patients with OA. Overexpression of Fibulin-5 significantly increased the expressions of ECM collagen II and aggrecan in chondrocytes, while decreasing the expressions of MMP-3 and MMP-13. In addition, Fibulin-5 reduced IL-1β-induced inflammation of chondrocytes, as well as expressions of IL-6, IL-8, and TNF-α. Overexpression of Fibulin-5 also reduced the activity of Wnt/β-catenin signaling pathway, and activation of Wnt/β-catenin signaling pathway attenuated the protective effects of Fibulin-5 on the ECM of chondrocytes.

Conclusions

Fibulin-5 can protect the ECM of chondrocytes and reduce the inflammatory response of chondrocytes by inhibiting the Wnt/β-catenin signaling pathway."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.org/dc/terms/identifier"doi:10.26355/eurrev_202005_21307"xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Lin L."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Gao S.J."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Zhang M.H."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Dong J.T."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Zhao J.B."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Gao J.B."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Zhao S.Q."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Jin L.P."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/author"Men X.Q."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/name"Eur Rev Med Pharmacol Sci"xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/pages"5249-5258"xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/title"Fibulin-5 protects the extracellular matrix of chondrocytes by inhibiting the Wnt/beta-catenin signaling pathway and relieves osteoarthritis."xsd:string
http://purl.uniprot.org/citations/32495858http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/32495858http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32495858
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