| http://purl.uniprot.org/citations/32499408 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32499408 | http://www.w3.org/2000/01/rdf-schema#comment | "In the fission yeast Schizosaccharomyces pombe, both RNAi machinery and RNAi-independent factors mediate transcriptional and posttranscriptional silencing and heterochromatin formation. Here, we show that the silencing of reporter genes at major native heterochromatic loci (centromeres, telomeres, mating-type locus and rDNA regions) and an artificially induced heterochromatin locus is alleviated in a fission yeast hsp90 mutant, hsp90-G84C Also, H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects. We further discovered that Hsp90 is required for stabilization or assembly of the RNA-induced transcriptional silencing (RITS) and Argonaute siRNA chaperone (ARC) RNAi effector complexes, the RNAi-independent factor Fft3, the shelterin complex subunit Poz1 and the Snf2/HDAC-containing repressor complex (SHREC). Our ChIP data suggest that Hsp90 regulates the efficient recruitment of the methyltransferase/ubiquitin ligase complex CLRC by shelterin to chromosome ends and targeting of the SHREC and Fft3 to mating type locus and/or rDNA region. Finally, our genetic analyses demonstrated that increased heterochromatin spreading restores silencing at subtelomeres in the hsp90-G84C mutant. Thus, this work uncovers a conserved factor critical for promoting RNAi-dependent and -independent heterochromatin assembly and gene silencing through stabilizing multiple effectors and effector complexes."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.244863"xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "He X."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "Sun L."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "Liu X.M."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "Li W.Z."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "Jin Q.W."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/author | "Yi Y.Y."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/pages | "jcs244863"xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/title | "The molecular chaperone Hsp90 regulates heterochromatin assembly through stabilizing multiple complexes in fission yeast."xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://purl.uniprot.org/core/volume | "133"xsd:string |
| http://purl.uniprot.org/citations/32499408 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32499408 |
| http://purl.uniprot.org/citations/32499408 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32499408 |
| http://purl.uniprot.org/uniprot/P41887#attribution-C79B793225EE024C6020A7F93F5FAF35 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/32499408 |
| http://purl.uniprot.org/uniprot/#_P41887-mappedCitation-32499408 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32499408 |
| http://purl.uniprot.org/uniprot/P41887 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32499408 |