| http://purl.uniprot.org/citations/32521021 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32521021 | http://www.w3.org/2000/01/rdf-schema#comment | "A striking feature of micro-RNAs is that they are often clustered in the genomes of animals. The functional and evolutionary consequences of this clustering remain obscure. Here, we investigated a micro-RNA cluster miR-6/5/4/286/3/309 that is conserved across drosophilid lineages. Small RNA sequencing revealed expression of this micro-RNA cluster in Drosophila melanogaster leg discs, and conditional overexpression of the whole cluster resulted in leg appendage shortening. Transgenic overexpression lines expressing different combinations of micro-RNA cluster members were also constructed. Expression of individual micro-RNAs from the cluster resulted in a normal wild-type phenotype, but either the expression of several ancient micro-RNAs together (miR-5/4/286/3/309) or more recently evolved clustered micro-RNAs (miR-6-1/2/3) can recapitulate the phenotypes generated by the whole-cluster overexpression. Screening of transgenic fly lines revealed downregulation of leg-patterning gene cassettes in generation of the leg-shortening phenotype. Furthermore, cell transfection with different combinations of micro-RNA cluster members revealed a suite of downstream genes targeted by all cluster members, as well as complements of targets that are unique for distinct micro-RNAs. Considered together, the micro-RNA targets and the evolutionary ages of each micro-RNA in the cluster demonstrate the importance of micro-RNA clustering, where new members can reinforce and modify the selection forces on both the cluster regulation and the gene regulatory network of existing micro-RNAs. Key words: micro-RNA, cluster, evolution."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.org/dc/terms/identifier | "doi:10.1093/molbev/msaa146"xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Zhong S."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Lau K.F."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Chan T.F."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Tobe S.S."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Bendena W.G."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Nong W."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Ferrier D.E.K."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Chu K.H."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Wong A.Y.P."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Hui J.H.L."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Zhe Q."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Cheung F.K.M."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Yiu W.C."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Lee I.H.T."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Wong N.W.Y."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Yip H.Y."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/author | "Yu C.W.C."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/name | "Mol Biol Evol"xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/pages | "2955-2965"xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/title | "Micro-RNA Clusters Integrate Evolutionary Constraints on Expression and Target Affinities: The miR-6/5/4/286/3/309 Cluster in Drosophila."xsd:string |
| http://purl.uniprot.org/citations/32521021 | http://purl.uniprot.org/core/volume | "37"xsd:string |