| http://purl.uniprot.org/citations/32544461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32544461 | http://www.w3.org/2000/01/rdf-schema#comment | "Bacteria and their toxins are associated with significant human morbidity and mortality. While a few bacterial toxins are well characterized, the mechanism of action for most toxins has not been elucidated, thereby limiting therapeutic advances. One such example is the highly potent pore-forming toxin, hemolysin BL (HBL), produced by the gram-positive pathogen Bacillus cereus. However, how HBL exerts its effects and whether it requires any host factors is unknown. Here, we describe an unbiased genome-wide CRISPR-Cas9 knockout screen that identified LPS-induced TNF-α factor (LITAF) as the HBL receptor. Using LITAF-deficient cells, a second, subsequent whole-genome CRISPR-Cas9 screen identified the LITAF-like protein CDIP1 as a second, alternative receptor. We generated LITAF-deficient mice, which exhibit marked resistance to lethal HBL challenges. This work outlines and validates an approach to use iterative genome-wide CRISPR-Cas9 screens to identify the complement of host factors exploited by bacterial toxins to exert their myriad biological effects."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.chom.2020.05.012"xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Liu C."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Liu S."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Zuo Z."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Jang J.Y."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Leppla S.H."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Sekine Y."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Finkel T."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Sastalla I."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/author | "Pirooznia M."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/name | "Cell Host Microbe"xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/pages | "402-410.e5"xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/title | "Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors."xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/32544461 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32544461 |
| http://purl.uniprot.org/citations/32544461 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32544461 |
| http://purl.uniprot.org/uniprot/#_E0CYR5-mappedCitation-32544461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32544461 |
| http://purl.uniprot.org/uniprot/#_F6X7Q6-mappedCitation-32544461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32544461 |
| http://purl.uniprot.org/uniprot/#_Q3UQV3-mappedCitation-32544461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32544461 |
| http://purl.uniprot.org/uniprot/#_Q9JLJ0-mappedCitation-32544461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32544461 |