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http://purl.uniprot.org/citations/32572884http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32572884http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To elucidate the relationship between microRNA-566 (miR-566) and prognosis in breast cancer (BC) and to clarify the influences of miR-566 and WNT6 in its locus region on BC progression.

Patients and methods

MiR-566 and WNT6 levels in 44 pairs of BC samples were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The influences of miR-566 on clinical features and prognosis in BC patients were analyzed. According to the differential expressions of miR-566 in the tested BC cell lines, MDA-MB-231 and MCF-7 cells were selected for generating miR-566 knockdown and overexpression models, respectively. Cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) and transwell assays were conducted to explore the role of miR-566 in BC cell functions. Besides, the regulatory interaction between miR-566 and its downstream gene WNT6 was assessed by performing Dual-Luciferase reporter assay. Finally, the co-regulation of miR-566 and WNT6 in BC cell functions was examined.

Results

MiR-566 was downregulated in BC tissues. BC patients with a low expression level of miR-566 were prone to suffering a large tumor size, advanced tumor grade, high incidence of lymphatic metastasis and poor prognosis. Overexpression of miR-566 weakened proliferative and migratory abilities in MCF-7 cells, whereas knockdown of miR-566 produced the opposite results in MDA-MB-231 cells. WNT6 was the target gene binding to miR-566, and they displayed a negative expression correlation in BC tissues. Regulatory effects of miR-566 on BC progression could be reversed by WNT6.

Conclusions

MiR-566 is closely related to tumor size, tumor grade, lymphatic metastasis and prognosis in BC. It protects the malignant progression of BC by negatively regulating WNT6."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.org/dc/terms/identifier"doi:10.26355/eurrev_202006_21514"xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Li T."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Zhang J."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Hu X.C."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Zhang M.M."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Wang L.P."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Wang B.Y."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Du Y.Q."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Tao Z.H."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/author"Zhao M.C."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/name"Eur Rev Med Pharmacol Sci"xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/pages"6185-6194"xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/title"MiR-566 protects the malignant progression of breast cancer by negatively regulating WNT6."xsd:string
http://purl.uniprot.org/citations/32572884http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/32572884http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32572884
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