| http://purl.uniprot.org/citations/32651375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32651375 | http://www.w3.org/2000/01/rdf-schema#comment | "DOCK (dedicator of cytokinesis) proteins are multidomain guanine nucleotide exchange factors (GEFs) for RHO GTPases that regulate intracellular actin dynamics. DOCK proteins share catalytic (DOCKDHR2) and membrane-associated (DOCKDHR1) domains. The structurally-related DOCK1 and DOCK2 GEFs are specific for RAC, and require ELMO (engulfment and cell motility) proteins for function. The N-terminal RAS-binding domain (RBD) of ELMO (ELMORBD) interacts with RHOG to modulate DOCK1/2 activity. Here, we determine the cryo-EM structures of DOCK2-ELMO1 alone, and as a ternary complex with RAC1, together with the crystal structure of a RHOG-ELMO2RBD complex. The binary DOCK2-ELMO1 complex adopts a closed, auto-inhibited conformation. Relief of auto-inhibition to an active, open state, due to a conformational change of the ELMO1 subunit, exposes binding sites for RAC1 on DOCK2DHR2, and RHOG and BAI GPCRs on ELMO1. Our structure explains how up-stream effectors, including DOCK2 and ELMO1 phosphorylation, destabilise the auto-inhibited state to promote an active GEF."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-020-17271-9"xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Barford D."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Chang L."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "McLaughlin S.H."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Yang J."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Smith M.J."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Boland A."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Cote J.F."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Jo C.H."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Abu-Thuraia A."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/author | "Killoran R.C."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/pages | "3464"xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/title | "Structure of the DOCK2-ELMO1 complex provides insights into regulation of the auto-inhibited state."xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/32651375 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32651375 |
| http://purl.uniprot.org/citations/32651375 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32651375 |
| http://purl.uniprot.org/uniprot/#_A4D1X5-mappedCitation-32651375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32651375 |
| http://purl.uniprot.org/uniprot/#_A0A2X0SFD3-mappedCitation-32651375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32651375 |
| http://purl.uniprot.org/uniprot/#_B3KXW9-mappedCitation-32651375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32651375 |
| http://purl.uniprot.org/uniprot/#_B3KY14-mappedCitation-32651375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32651375 |