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http://purl.uniprot.org/citations/32653671http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32653671http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

DNA repair proteins have emerged as potential predictors for immunotherapy response alongside PD-L1 expression, tumor-infiltrating lymphocytes (TILs) and tumor mutational burden. We analyzed expression of PD-L1, TILs count and expression of the homologous recombination (HR) protein RAD51, as potential prognostic factors in patients with resected non-small-cell lung carcinoma (NSCLC).

Materials and methods

Discovery set included 96 NSCLC patients from the University Hospital Olomouc (Czech Republic) and a replication set included 1109 NSCLC patients from University Hospital Zurich (Switzerland). Tissue microarrays (TMAs) were stained using the automated staining platform Ventana Benchmark Ultra with antibodies against RAD51,CD3, CD8, CD68 and PD-L1.

Results

Loss of nuclear RAD51 protein was associated with high TILs (r=-0.25, p = 0.01) and PD-L1 status (10.6 vs. 2.4 %, p = 0.012) in patients receiving neoadjuvant chemo-/radiotherapy (CT/RT). In silico analysis from the TCGA data set showed a negative relationship between RAD51 mRNA expression and CD45 (r = ‒0.422, p < 0.0001), CD68 (r = ‒0.326, p < 0.001), CD3 (r = ‒0.266, p < 0.001) and CD8 (r = ‒0.102, p < 0.001). RAD51 low/PD-L1 high patients were clustered as separate entity in the replication set and in TCGA dataset. High TILs status was significantly associated with improved OS in the replication set (unadjusted HR = 0.57, 95 % CI 0.42-0.76, p < 0.001). Similar results have been seen for CD3, CD8 and CD68.

Conclusions

In conclusion, RAD51 nuclear loss is weakly associated with increased TILs and high PD-L1 at the time of surgery in curatively resected NSCLC and after prior exposure to neoadjuvant chemo- or radiotherapy. Both high TILs and RAD51 nuclear loss were confirmed as independent prognostic factors in curatively resected NSCLC."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.org/dc/terms/identifier"doi:10.1016/j.lungcan.2020.06.025"xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Hajduch M."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Skarda J."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Soltermann A."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Shani I."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Vojta P."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Kolar Z."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Stahel R."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Kolek V."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Joerger M."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Weder W."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Gachechiladze M."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Uberall I."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Smickova P."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Rulle U."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Skanderova D."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/author"Vbrkova J."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/name"Lung Cancer"xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/pages"30-38"xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/title"Prognostic value of tumor-infiltrating lymphocytes (TILs) and their association with PD-L1 expression and DNA repair protein RAD51 in patients with resected non-small cell lung carcinoma."xsd:string
http://purl.uniprot.org/citations/32653671http://purl.uniprot.org/core/volume"147"xsd:string
http://purl.uniprot.org/citations/32653671http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/32653671