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Background

Genetically encoded fluorescent proteins are often used to label proteins and study protein function and localization in vivo. Traditional cloning methods mediated by restriction digestion and ligation are time-consuming and sometimes difficult due to the lack of suitable restriction sites. Invitrogen developed the Gateway cloning system based on the site-specific DNA recombination, which allows for digestion-free cloning. Most gateway destination vectors available for use in plants employ either the 35S or ubiquitin promoters, which confer high-level, ubiquitous expression. There are far fewer options for moderate, cell-type specific expression.

Results

Here we report on the construction of a Gateway-compatible cloning system (SWU vectors) to rapidly tag various proteins and express them in a cell-type specific manner in plants. We tested the SWU vectors using the HISTONE (H2B) coding sequence in stable transgenic plants.

Conclusions

The SWU vectors are a valuable tool for low cost, high efficiency functional analysis of proteins of interest in specific cell types in the Arabidopsis root."xsd:string
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http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/author"Liang H."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/author"Li X."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/author"Wu S."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/author"Zhao Y."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/author"Gallagher K.L."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/name"Plant Methods"xsd:string
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http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/title"Gateway-compatible vectors for functional analysis of proteins in cell type specific manner."xsd:string
http://purl.uniprot.org/citations/32655679http://purl.uniprot.org/core/volume"16"xsd:string
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