| http://purl.uniprot.org/citations/32754202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32754202 | http://www.w3.org/2000/01/rdf-schema#comment | "The vacuolar ATPases (V-ATPases) are ATP-dependent proton pumps that play vital roles in eukaryotic cells. Insect V-ATPases are required in nearly all epithelial tissues to regulate a multiplicity of processes including receptor-mediated endocytosis, protein degradation, fluid secretion, and neurotransmission. Composed of fourteen different subunits, several V-ATPase subunits exist in distinct isoforms to perform cell type specific functions. The 100 kD a subunit (Vha100) of V-ATPases are encoded by a family of five genes in Drosophila, but their assignments are not fully understood. Here we report an experimental survey of the Vha100 gene family during Drosophila wing development. A combination of CRISPR-Cas9 mutagenesis, somatic clonal analysis and in vivo RNAi assays is used to characterize the requirement of Vha100 isoforms, and mutants of Vha100-2, Vha100-3, Vha100-4, and Vha100-5 genes were generated. We show that Vha100-3 and Vha100-5 are dispensable for fly development, while Vha100-1 is not critically required in the wing. As for the other two isoforms, we find that Vha100-2 regulates wing cuticle maturation, while Vha100-4 is the single isoform involved in developmental patterning. More specifically, Vha100-4 is required for proper activation of the Wingless signaling pathway during fly wing development. Interestingly, we also find a specific genetic interaction between Vha100-1 and Vha100-4 during wing development. Our results revealed the distinct roles of Vha100 isoforms during insect wing development, providing a rationale for understanding the diverse roles of V-ATPases."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.org/dc/terms/identifier | "doi:10.3389/fgene.2020.00723"xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/author | "Chen Y."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/author | "Jiang N."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/author | "Shen J."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/author | "Mo D."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/name | "Front Genet"xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/pages | "723"xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/title | "Investigation of Isoform Specific Functions of the V-ATPase a Subunit During Drosophila Wing Development."xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/32754202 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32754202 |
| http://purl.uniprot.org/citations/32754202 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32754202 |
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| http://purl.uniprot.org/uniprot/#_A1ZBF7-mappedCitation-32754202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32754202 |
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