| http://purl.uniprot.org/citations/3275612 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3275612 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3275612 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/3275612 | http://www.w3.org/2000/01/rdf-schema#comment | "The rff genes of Salmonella typhimurium include structural genes for enzymes involved in the conversion of UDP N-acetyl-D-glucosamine (UDP-GlcNAc) to UDP N-acetyl-D-mannosaminuronic acid (UDP-ManNAcA), the donor of ManNAcA residues in enterobacterial common antigen (ECA) synthesis. An rff mutation (rff-726) of Escherichia coli has been described (U. Meier and H. Mayer, J. Bacteriol. 163:756-762, 1985) that abolished ECA synthesis but which did not affect the synthesis of UDP-ManNAcA or any other components of ECA. The nature of the enzymatic defect resulting from the rff-726 lesion was investigated in the present study. The in vitro synthesis of GlcNAc-pyrophosphorylundecaprenol (lipid I), an early intermediate in ECA synthesis, was demonstrated by using membranes prepared from a mutant of E. coli possessing the rff-726 lesion. However, in vitro synthesis of the next lipid-linked intermediate in the biosynthetic sequence, ManNAcA-GlcNAc-pyrophosphorylundecaprenol (lipid II), was severely impaired. Transduction of wild-type rff genes into the mutant restored the ability to synthesize both lipid II and ECA as determined by in vitro assay and Western blot (immunoblot) analyses done with anti-ECA monoclonal antibody, respectively. Our results are consistent with the conclusion that the rff-726 mutation is located in the structural gene for the transferase that catalyzes the transfer of ManNAcA from UDP-ManNAcA to lipid I."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.170.1.228-233.1988"xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.170.1.228-233.1988"xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Ward S."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Ward S."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Rick P.D."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Rick P.D."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Mayer H."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Mayer H."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Barr K."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Barr K."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Meier-Dieter U."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/author | "Meier-Dieter U."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/pages | "228-233"xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/pages | "228-233"xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/title | "Characterization of an Escherichia coli rff mutant defective in transfer of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-linked intermediate involved in enterobacterial common antigen synthesis."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/title | "Characterization of an Escherichia coli rff mutant defective in transfer of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-linked intermediate involved in enterobacterial common antigen synthesis."xsd:string |
| http://purl.uniprot.org/citations/3275612 | http://purl.uniprot.org/core/volume | "170"xsd:string |