| http://purl.uniprot.org/citations/3275872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3275872 | http://www.w3.org/2000/01/rdf-schema#comment | "STE3 mRNA is present only in Saccharomyces cerevisiae alpha cells, not in a or a/alpha cells, and the transcript level increases about fivefold when cells are treated with a-factor mating pheromone. Deletions in the 5' noncoding region of STE3 defined a 43-base-pair (bp) upstream activation sequence (UAS) that can impart both modes of regulation to a CYC1-lacZ fusion when substituted for the native CYC1 UAS. UAS activity required the alpha 1 product of MAT alpha, which is known to be required for transcription of alpha-specific genes. A chromosomal deletion that removed only 14 bp of the STE3 UAS reduced STE3 transcript levels 50-to 100-fold, indicating that the UAS is essential for expression. The STE3 UAS shares a 26-bp homology with the 5' noncoding sequences of the only other known alpha-specific genes, MF alpha 1 and MF alpha 2. We view the homology as having two components--a nearly palindromic 16-bp "P box" and an adjacent 10-bp "Q box." A synthetic STE3 P box was inactive as a UAS; a perfect palindrome P box was active in all three cell types. We propose that the P box is the binding site for a transcription activator, but that alpha 1 acting via the Q box is required for this activator to bind to the imperfect P boxes of alpha-specific genes. Versions of the P box are also found upstream of a-specific genes, within the binding sites of the repressor alpha 2 encoded by MAT alpha. Thus, the products of MAT alpha may render gene expression alpha or a-specific by controlling access of the same transcription activator to its binding site, the P box."xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.8.1.309-320.1988"xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/author | "Sprague G.F. Jr."xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/author | "Hagen D.C."xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/author | "Jarvis E.E."xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/pages | "309-320"xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/title | "Identification of a DNA segment that is necessary and sufficient for alpha-specific gene control in Saccharomyces cerevisiae: implications for regulation of alpha-specific and a-specific genes."xsd:string |
| http://purl.uniprot.org/citations/3275872 | http://purl.uniprot.org/core/volume | "8"xsd:string |
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