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http://purl.uniprot.org/citations/3278689http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3278689http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3278689http://www.w3.org/2000/01/rdf-schema#comment"The nucleotide sequence of a 1.4-kilobase DNA fragment containing the alpha-acetolactate decarboxylase gene of Enterobacter aerogenes was determined. The sequence contains an entire protein-coding region of 780 nucleotides which encodes an alpha-acetolactate decarboxylase of 260 amino acids. The DNA sequence coding for alpha-acetolactate decarboxylase was placed under the control of the alcohol dehydrogenase I promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both S. cerevisiae and Escherichia coli. Brewer's yeast cells transformed by this plasmid showed alpha-acetolactate decarboxylase activity and were used in laboratory-scale fermentation experiments. These experiments revealed that the diacetyl concentration in wort fermented by the plasmid-containing yeast strain was significantly lower than that in wort fermented by the parental strain. These results indicated that the alpha-acetolactate decarboxylase activity produced by brewer's yeast cells degraded alpha-acetolactate and that this degradation caused a decrease in diacetyl production."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.org/dc/terms/identifier"doi:10.1128/aem.54.1.38-42.1988"xsd:string
http://purl.uniprot.org/citations/3278689http://purl.org/dc/terms/identifier"doi:10.1128/aem.54.1.38-42.1988"xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Fujii T."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Fujii T."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Inoue T."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Inoue T."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Kondo K."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Kondo K."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Shimizu F."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Shimizu F."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Sone H."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Sone H."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Tanaka J."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/author"Tanaka J."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/date"1988"xsd:gYear
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/date"1988"xsd:gYear
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/name"Appl. Environ. Microbiol."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/name"Appl. Environ. Microbiol."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/pages"38-42"xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/pages"38-42"xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/title"Nucleotide sequence and expression of the Enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast."xsd:string
http://purl.uniprot.org/citations/3278689http://purl.uniprot.org/core/title"Nucleotide sequence and expression of the Enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast."xsd:string