http://purl.uniprot.org/citations/32876065 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/32876065 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/32876065 | http://www.w3.org/2000/01/rdf-schema#comment | "The crystal structure of a bacteriophage T4 early gene product, Spackle, was determined by native sulfur single-wavelength anomalous diffraction (SAD) phasing using synchrotron radiation and was refined to 1.52 Å resolution. The structure shows that Spackle consists of a bundle of five α-helices, forming a relatively flat disc-like overall shape. Although Spackle forms a dimer in the crystal, size-exclusion chromatography with multi-angle light scattering shows that it is monomeric in solution. Mass spectrometry confirms that purified mature Spackle lacks the amino-terminal signal peptide and contains an intramolecular disulfide bond, consistent with its proposed role in the periplasm of T4 phage-infected Escherichia coli cells. The surface electrostatic potential of Spackle shows a strikingly bipolar charge distribution, suggesting a possible mode of membrane association and inhibition of the tail lysozyme activity in T4 bacteriophage superinfection exclusion."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.org/dc/terms/identifier | "doi:10.1107/s2059798320010979"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.org/dc/terms/identifier | "doi:10.1107/s2059798320010979"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Shi K."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Shi K."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Banerjee S."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Banerjee S."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Aihara H."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Aihara H."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Kurniawan F."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Kurniawan F."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Moeller N.H."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/author | "Moeller N.H."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/pages | "899-904"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/pages | "899-904"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/title | "Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/title | "Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing."xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/volume | "76"xsd:string |
http://purl.uniprot.org/citations/32876065 | http://purl.uniprot.org/core/volume | "76"xsd:string |