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| http://purl.uniprot.org/citations/32879423 | http://www.w3.org/2000/01/rdf-schema#comment | "In higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the redox modulation, we used single-particle cryo-EM to determine the structures of spinach chloroplast ATP synthase in both reduced and oxidized states. The disulfide linkage of the oxidized γ subunit introduces a torsional constraint to stabilize the two β hairpin structures. Once reduced, free cysteines alleviate this constraint, resulting in a concerted motion of the enzyme complex and a smooth transition between rotary states to facilitate the ATP synthesis. We added an uncompetitive inhibitor, tentoxin, in the reduced sample to limit the flexibility of the enzyme and obtained high-resolution details. Our cryo-EM structures provide mechanistic insight into the redox modulation of the energy regulation activity of chloroplast ATP synthase."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.org/dc/terms/identifier | "doi:10.1038/s42003-020-01221-8"xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/author | "Fromme P."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/author | "Williams D."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/author | "Yang J.H."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/author | "Kandiah E."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/author | "Chiu P.L."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/name | "Commun Biol"xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/pages | "482"xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/title | "Structural basis of redox modulation on chloroplast ATP synthase."xsd:string |
| http://purl.uniprot.org/citations/32879423 | http://purl.uniprot.org/core/volume | "3"xsd:string |
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