| http://purl.uniprot.org/citations/32890404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32890404 | http://www.w3.org/2000/01/rdf-schema#comment | "The ribosomal stalk proteins, RPLP1 and RPLP2 (RPLP1/2), which form the ancient ribosomal stalk, were discovered decades ago but their functions remain mysterious. We had previously shown that RPLP1/2 are exquisitely required for replication of dengue virus (DENV) and other mosquito-borne flaviviruses. Here, we show that RPLP1/2 function to relieve ribosome pausing within the DENV envelope coding sequence, leading to enhanced protein stability. We evaluated viral and cellular translation in RPLP1/2-depleted cells using ribosome profiling and found that ribosomes pause in the sequence coding for the N-terminus of the envelope protein, immediately downstream of sequences encoding two adjacent transmembrane domains (TMDs). We also find that RPLP1/2 depletion impacts a ribosome density for a small subset of cellular mRNAs. Importantly, the polarity of ribosomes on mRNAs encoding multiple TMDs was disproportionately affected by RPLP1/2 knockdown, implying a role for RPLP1/2 in multi-pass transmembrane protein biogenesis. These analyses of viral and host RNAs converge to implicate RPLP1/2 as functionally important for ribosomes to elongate through ORFs encoding multiple TMDs. We suggest that the effect of RPLP1/2 at TMD associated pauses is mediated by improving the efficiency of co-translational folding and subsequent protein stability."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkaa717"xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/author | "Shah P."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/author | "Bradrick S.S."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/author | "Garcia-Blanco M.A."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/author | "Campos R.K."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/author | "Wijeratne H.R.S."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/pages | "9872-9885"xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/title | "Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins."xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/32890404 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32890404 |
| http://purl.uniprot.org/citations/32890404 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32890404 |
| http://purl.uniprot.org/uniprot/#_P05386-mappedCitation-32890404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/#_P05387-mappedCitation-32890404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32890404 |
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| http://purl.uniprot.org/uniprot/#_Q6ICQ4-mappedCitation-32890404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/#_Q7Z612-mappedCitation-32890404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/P05386 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/Q6ICQ4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/P05387 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/Q6FG99 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32890404 |
| http://purl.uniprot.org/uniprot/Q7Z612 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32890404 |