| http://purl.uniprot.org/citations/32927637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32927637 | http://www.w3.org/2000/01/rdf-schema#comment | "Porcine reproductive and respiratory syndrome virus (PRRSV) has disrupted the global swine industry since the 1980s. PRRSV-host interactions are largely still unknown but may involve host ISG15 protein. In this study, we developed a monoclonal antibody (Mab-3D5E6) specific for swine ISG15 (sISG15) by immunizing mice with recombinant sISG15. A sandwich enzyme-linked immunosorbent assay (ELISA) incorporating this sISG15-specific Mab was developed to detect sISG15 and provided a lower limit of sISG15 detection of 200 pg/mL. ELISA results demonstrated that infection of porcine alveolar macrophages (PAMs) with low-virulence or attenuated PRRSV vaccine strains induced intracellular ISG15 expression that was independent of type I IFN production, while PAMs infection with a PRRSV vaccine strain promoted extracellular ISG15 secretion from infected PAMs. Conversely, the addition of recombinant sISG15 to PAMs mimicked natural extracellular ISG15 effects whereby sISG15 functioned as a cytokine by activating PAMs. Once activated, PAMs could inhibit PRRSV replication and resist infection with PRRSV vaccine strain TJM. In summary, a sandwich ELISA incorporating homemade anti-ISG15 Mab detected ISG15 secretion induced by PAMs infection with a PRRSV vaccine strain. Recombinant ISG15 added to cells exhibited cytokine-like activity that stimulated PAMs to assume an anti-viral state that enabled them to inhibit PRRSV replication and resist viral infection."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.org/dc/terms/identifier | "doi:10.3390/v12091009"xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Nan Y."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Zhao G."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Zhao B."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/author | "Shi B."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/name | "Viruses"xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/pages | "E1009"xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/title | "PRRSV Vaccine Strain-Induced Secretion of Extracellular ISG15 Stimulates Porcine Alveolar Macrophage Antiviral Response against PRRSV."xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/32927637 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32927637 |
| http://purl.uniprot.org/citations/32927637 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32927637 |
| http://purl.uniprot.org/uniprot/#_A0A8D1LSM4-mappedCitation-32927637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32927637 |
| http://purl.uniprot.org/uniprot/#_A0A287AB58-mappedCitation-32927637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32927637 |
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| http://purl.uniprot.org/uniprot/#_B2ZDZ2-mappedCitation-32927637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32927637 |
| http://purl.uniprot.org/uniprot/B2LUG8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32927637 |
| http://purl.uniprot.org/uniprot/B2ZDZ2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32927637 |
| http://purl.uniprot.org/uniprot/A0A287AB58 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32927637 |
| http://purl.uniprot.org/uniprot/A0A8D1LSM4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32927637 |