| http://purl.uniprot.org/citations/32928959 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32928959 | http://www.w3.org/2000/01/rdf-schema#comment | "Punctin/MADD-4, a member of the ADAMTSL extracellular matrix protein family, was identified as an anterograde synaptic organizer in the nematode Caenorhabditis elegans. At GABAergic neuromuscular junctions, the short isoform MADD-4B binds the ectodomain of neuroligin NLG-1, itself a postsynaptic organizer of inhibitory synapses. To identify the molecular bases of their partnership, we generated recombinant forms of the two proteins and carried out a comprehensive biochemical and biophysical study of their interaction, complemented by an in vivo localization study. We show that spontaneous proteolysis of MADD-4B first generates a shorter N-MADD-4B form, which comprises four thrombospondin (TSP) domains and one Ig-like domain and binds NLG-1. A second processing event eliminates the C-terminal Ig-like domain along with the ability of N-MADD-4B to bind NLG-1. These data identify the Ig-like domain as the primary determinant for N-MADD-4B interaction with NLG-1 in vitro We further demonstrate in vivo that this Ig-like domain is essential, albeit not sufficient per se, for efficient recruitment of GABAA receptors at GABAergic synapses in C. elegans The interaction of N-MADD-4B with NLG-1 is also disrupted by heparin, used as a surrogate for the extracellular matrix component, heparan sulfate. High-affinity binding of heparin/heparan sulfate to the Ig-like domain may proceed from surface charge complementarity, as suggested by homology three-dimensional modeling. These data point to N-MADD-4B processing and cell-surface proteoglycan binding as two possible mechanisms to regulate the interaction between MADD-4B and NLG-1 at GABAergic synapses."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra120.014591"xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Bourne Y."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Fourquet P."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Marchot P."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Zhou X."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Tu H."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Pinan-Lucarre B."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Bessereau J.L."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Mansuelle P."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Delauzun V."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/author | "Platsaki S."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/pages | "16267-16279"xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/title | "The Ig-like domain of Punctin/MADD-4 is the primary determinant for interaction with the ectodomain of neuroligin NLG-1."xsd:string |
| http://purl.uniprot.org/citations/32928959 | http://purl.uniprot.org/core/volume | "295"xsd:string |
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| http://purl.uniprot.org/uniprot/#_G5EED7-mappedCitation-32928959 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32928959 |