| http://purl.uniprot.org/citations/32949667 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32949667 | http://www.w3.org/2000/01/rdf-schema#comment | "The proliferation and differentiation of NSCs are regulated by miRNAs. This study investigated the role of miR-374a-5p in the proliferation and differentiation of ReNcell VM cells. ReNcell VM cells were transfected with miR-374a-5p mimic, miR-374a-5p inhibitor and Hes1, respectively. Cell proliferation was detected by clone formation assay. Target gene for miR-374a-5p was predicted by TargetScan and confirmed by dual-luciferase reporter. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were performed to detect the expressions of relative genes. After culturing the cells in differentiation medium, the ReNcell VM cells differentiated into βIII-tubulin (Tuj1)-positive neurons and GFAP-positive astrocytes. The miR-374a-5p expression was increased as the cells continued to differentiate. Hes1, which was predicted to be the target gene for miR-374a-5p, was low-expressed during cell differentiation. The miR-374a-5p mimic decreased cell clones, inhibited the expressions of ki-67 and Nestin, but increased those of Tuj1 and GFAP. However, miR-374a-5p inhibitor produced the opposite effects to miR-374a-5p mimic. Hes1 increased the expressions of ki-67 and Nestin, but decreased those of Tuj1 and GFAP, moreover, Hes1 reversed the role of miR-374a-5p mimic. MiR-374a-5p inhibited the proliferation of Rencell VM cells and promoted the differentiation of NSCs by reducing the Hes1 expression."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.neures.2020.09.002"xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "He Z."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "Xu J."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "Wu Z."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "Xu Z."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/author | "Yang B."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/name | "Neurosci Res"xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/pages | "99-105"xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/title | "High expression of miR-374a-5p inhibits the proliferation and promotes differentiation of Rencell VM cells by targeting Hes1."xsd:string |
| http://purl.uniprot.org/citations/32949667 | http://purl.uniprot.org/core/volume | "170"xsd:string |
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