| http://purl.uniprot.org/citations/32958564 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32958564 | http://www.w3.org/2000/01/rdf-schema#comment | "Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles via the recognition of motifs based on Tyr or di-Leu in their cytoplasmic tails. However, in plants, very little is known about how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis (Arabidopsis thaliana), the brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) undergoes endocytosis, which depends on clathrin and AP-2. Here, we demonstrate that BRI1 binds directly to the medium AP-2 subunit (AP2M). The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed Tyr-based endocytic motifs. The Tyr-to-Phe substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional Tyr motifs also operates in plants."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.org/dc/terms/identifier | "doi:10.1105/tpc.20.00384"xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Liu D."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Kumar R."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Friml J."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Russinova E."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Zhao X."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Wang P."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Van Damme D."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Vert G."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Vanhoutte I."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Martins S."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Johnson A.J."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Bender K.W."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Yperman K."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Siao W."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/author | "Claus L.A.N."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/name | "Plant Cell"xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/pages | "3598-3612"xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/title | "Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyr-Based Motif."xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/32958564 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32958564 |
| http://purl.uniprot.org/citations/32958564 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32958564 |