| http://purl.uniprot.org/citations/32958604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32958604 | http://www.w3.org/2000/01/rdf-schema#comment | "Tetraspanins are eukaryotic membrane proteins that contribute to a variety of signaling processes by organizing partner-receptor molecules in the plasma membrane. How tetraspanins bind and cluster partner receptors into tetraspanin-enriched microdomains is unknown. Here, we present crystal structures of the large extracellular loop of CD9 bound to nanobodies 4C8 and 4E8 and, the cryo-EM structure of 4C8-bound CD9 in complex with its partner EWI-F. CD9-EWI-F displays a tetrameric arrangement with two central EWI-F molecules, dimerized through their ectodomains, and two CD9 molecules, one bound to each EWI-F transmembrane helix through CD9-helices h3 and h4. In the crystal structures, nanobodies 4C8 and 4E8 bind CD9 at loops C and D, which is in agreement with the 4C8 conformation in the CD9-EWI-F complex. The complex varies from nearly twofold symmetric (with the two CD9 copies nearly anti-parallel) to ca. 50° bent arrangements. This flexible arrangement of CD9-EWI-F with potential CD9 homo-dimerization at either end provides a "concatenation model" for forming short linear or circular assemblies, which may explain the occurrence of tetraspanin-enriched microdomains."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.org/dc/terms/identifier | "doi:10.26508/lsa.202000883"xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Lutz M."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Gros P."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Pos W."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Kroon-Batenburg L.M."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Oosterheert W."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Doulkeridou S."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Pearce N.M."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "van Bergen En Henegouwen P.M."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Neviani V."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Manshande J."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/author | "Xenaki K.T."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/name | "Life Sci Alliance"xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/pages | "e202000883"xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/title | "Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F."xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/32958604 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32958604 |
| http://purl.uniprot.org/citations/32958604 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32958604 |
| http://purl.uniprot.org/uniprot/#_P21926-mappedCitation-32958604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32958604 |
| http://purl.uniprot.org/uniprot/P21926 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32958604 |