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http://purl.uniprot.org/citations/32958672http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32958672http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/32958672http://www.w3.org/2000/01/rdf-schema#comment"While near-cognate codons are frequently used for translation initiation in eukaryotes, their efficiencies are usually low (<10% compared to an AUG in optimal context). Here, we describe a rare case of highly efficient near-cognate initiation. A CUG triplet located in the 5' leader of POLG messenger RNA (mRNA) initiates almost as efficiently (∼60 to 70%) as an AUG in optimal context. This CUG directs translation of a conserved 260-triplet-long overlapping open reading frame (ORF), which we call POLGARF (POLG Alternative Reading Frame). Translation of a short upstream ORF 5' of this CUG governs the ratio between POLG (the catalytic subunit of mitochondrial DNA polymerase) and POLGARF synthesized from a single POLG mRNA. Functional investigation of POLGARF suggests a role in extracellular signaling. While unprocessed POLGARF localizes to the nucleoli together with its interacting partner C1QBP, serum stimulation results in rapid cleavage and secretion of a POLGARF C-terminal fragment. Phylogenetic analysis shows that POLGARF evolved ∼160 million y ago due to a mammalian-wide interspersed repeat (MIR) transposition into the 5' leader sequence of the mammalian POLG gene, which became fixed in placental mammals. This discovery of POLGARF unveils a previously undescribed mechanism of de novo protein-coding gene evolution."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.org/dc/terms/identifier"doi:10.1073/pnas.2001433117"xsd:string
http://purl.uniprot.org/citations/32958672http://purl.org/dc/terms/identifier"doi:10.1073/pnas.2001433117"xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Atkins J.F."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Atkins J.F."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Loughran G."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Loughran G."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Serebryakova M.V."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Serebryakova M.V."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Shatsky I.N."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Shatsky I.N."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Andreev D.E."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Andreev D.E."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Baranov P.V."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Baranov P.V."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Datskevich P.N."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Datskevich P.N."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Kiniry S.J."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Kiniry S.J."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Kovalchuk S.I."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Kovalchuk S.I."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Michel A.M."xsd:string
http://purl.uniprot.org/citations/32958672http://purl.uniprot.org/core/author"Michel A.M."xsd:string
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