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http://purl.uniprot.org/citations/33109222http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33109222http://www.w3.org/2000/01/rdf-schema#comment"

Background

Lung cancer (LC) is a malignant tumor originating in the bronchial mucosa or gland of the lung. Circular RNAs (circRNAs) are proved to be key regulators of tumor progression. However, the regulatory effect of circ_0001421 on lung cancer tumorigenesis remains unclear.

Methods

The expression levels of circ_0001421, microRNA-4677-3p (miR-4677-3p) and cell division cycle associated 3 (CDCA3) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methyl thiazolyl tetrazolium (MTT), Transwell and Tumor formation assays were performed to explore the role of circ_0001421 in LC. Glucose consumption and lactate production were examined by a Glucose assay kit and a Lactic Acid assay kit. Western blot was utilized to examine the protein levels of Hexokinase 2 (HK2) and CDCA3. The interaction between miR-4677-3p and circ_0001421 or CDCA3 was confirmed by dual-luciferase reporter assay.

Results

Circ_0001421 was increased in LC tissues and cells, and knockdown of circ_0001421 repressed cell proliferation, migration, invasion and glycolysis in vitro. Meanwhile, circ_0001421 knockdown inhibited LC tumor growth in vivo. Mechanistically, circ_0001421 could bind to miR-4677-3p, and CDCA3 was a target of miR-4677-3p. Rescue assays manifested that silencing miR-4677-3p or CDCA3 overexpression reversed circ_0001421 knockdown-mediated suppression on cell proliferation, migration, invasion and glycolysis in LC cells.

Conclusion

Circ_0001421 promoted cell proliferation, migration, invasion and glycolysis in LC by regulating the miR-4677-3p/CDCA3 axis, which providing a new mechanism for LC tumor progression."xsd:string
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http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/author"Chen H."xsd:string
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/author"Hu H."xsd:string
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/author"Jiang Y."xsd:string
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http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/author"Qiu L."xsd:string
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/author"Xu J."xsd:string
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/author"Zhang K."xsd:string
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/name"Diagn Pathol"xsd:string
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http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/title"Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3."xsd:string
http://purl.uniprot.org/citations/33109222http://purl.uniprot.org/core/volume"15"xsd:string
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