| http://purl.uniprot.org/citations/33122194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33122194 | http://www.w3.org/2000/01/rdf-schema#comment | "Numerous iron-sulfur (Fe-S) proteins with diverse functions are present in the matrix and respiratory chain complexes of mitochondria. Although [4Fe-4S] clusters are the most common type of Fe-S cluster in mitochondria, the molecular mechanism of [4Fe-4S] cluster assembly and insertion into target proteins by the mitochondrial iron-sulfur cluster (ISC) maturation system is not well-understood. Here we report a detailed characterization of two late-acting Fe-S cluster-carrier proteins from Arabidopsis thaliana, NFU4 and NFU5. Yeast two-hybrid and bimolecular fluorescence complementation studies demonstrated interaction of both the NFU4 and NFU5 proteins with the ISCA class of Fe-S carrier proteins. Recombinant NFU4 and NFU5 were purified as apo-proteins after expression in Escherichia coliIn vitro Fe-S cluster reconstitution led to the insertion of one [4Fe-4S]2+ cluster per homodimer as determined by UV-visible absorption/CD, resonance Raman and EPR spectroscopy, and analytical studies. Cluster transfer reactions, monitored by UV-visible absorption and CD spectroscopy, showed that a [4Fe-4S]2+ cluster-bound ISCA1a/2 heterodimer is effective in transferring [4Fe-4S]2+ clusters to both NFU4 and NFU5 with negligible back reaction. In addition, [4Fe-4S]2+ cluster-bound ISCA1a/2, NFU4, and NFU5 were all found to be effective [4Fe-4S]2+ cluster donors for maturation of the mitochondrial apo-aconitase 2 as assessed by enzyme activity measurements. The results demonstrate rapid, unidirectional, and quantitative [4Fe-4S]2+ cluster transfer from ISCA1a/2 to NFU4 or NFU5 that further delineates their respective positions in the plant ISC machinery and their contributions to the maturation of client [4Fe-4S] cluster-containing proteins."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra120.015726"xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/author | "Johnson M.K."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/author | "Couturier J."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/author | "Vignols F."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/author | "Rouhier N."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/author | "Azam T."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/author | "Przybyla-Toscano J."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/pages | "18367-18378"xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/title | "[4Fe-4S] cluster trafficking mediated by Arabidopsis mitochondrial ISCA and NFU proteins."xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://purl.uniprot.org/core/volume | "295"xsd:string |
| http://purl.uniprot.org/citations/33122194 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33122194 |
| http://purl.uniprot.org/citations/33122194 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33122194 |
| http://purl.uniprot.org/uniprot/#_F4IWC5-mappedCitation-33122194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33122194 |
| http://purl.uniprot.org/uniprot/#_Q9C8J2-mappedCitation-33122194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33122194 |
| http://purl.uniprot.org/uniprot/#_Q9LIG6-mappedCitation-33122194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33122194 |
| http://purl.uniprot.org/uniprot/F4IWC5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33122194 |
| http://purl.uniprot.org/uniprot/Q9C8J2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33122194 |
| http://purl.uniprot.org/uniprot/Q9LIG6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33122194 |