| http://purl.uniprot.org/citations/33144582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33144582 | http://www.w3.org/2000/01/rdf-schema#comment | "Ribosomes stalled during translation must be rescued to replenish the pool of translation-competent ribosomal subunits. Bacterial alternative rescue factor B (ArfB) releases nascent peptides from ribosomes stalled on mRNAs truncated at the A site, allowing ribosome recycling. Prior structural work revealed that ArfB recognizes such ribosomes by inserting its C-terminal α-helix into the vacant mRNA tunnel. In this work, we report that ArfB can efficiently recognize a wider range of mRNA substrates, including longer mRNAs that extend beyond the A-site codon. Single-particle cryo-EM unveils that ArfB employs two modes of function depending on the mRNA length. ArfB acts as a monomer to accommodate a shorter mRNA in the ribosomal A site. By contrast, longer mRNAs are displaced from the mRNA tunnel by more than 20 Å and are stabilized in the intersubunit space by dimeric ArfB. Uncovering distinct modes of ArfB function resolves conflicting biochemical and structural studies, and may lead to re-examination of other ribosome rescue pathways, whose functions depend on mRNA lengths."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-020-19370-z"xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/author | "Demo G."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/author | "Korostelev A.A."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/author | "Madireddy R."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/author | "Svidritskiy E."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/author | "Carbone C.E."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/pages | "5552"xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/title | "ArfB can displace mRNA to rescue stalled ribosomes."xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/33144582 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33144582 |
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