| http://purl.uniprot.org/citations/33146944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33146944 | http://www.w3.org/2000/01/rdf-schema#comment | "Chiral cyanohydrins are useful intermediates in the pharmaceutical and agricultural industries. In nature, hydroxynitrile lyases (HNLs) are a kind of elegant tool for enantioselective hydrocyanation of carbonyl compounds. However, currently available methods for demonstrating hydrocyanation are still stalled at precise, but low-throughput, GC or HPLC analyses. Herein, we report a chromogenic high-throughput screening (HTS) method that is feasible for the cyanohydrin synthesis reaction. This method was highly anti-interference and sensitive, and could be used to directly profile the substrate scope of HNLs either in cell-free extract or fermentation clear broth. This HTS method was also validated by generating new variants of PcHNL5 that presented higher catalytic efficiency and stronger acidic tolerance in variant libraries."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.202000658"xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Hong R."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Lin Z."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Jia Q."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Zheng Y.C."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Xu J.H."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Yu H.L."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/author | "Ding L.Y."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/name | "Chembiochem"xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/pages | "996-1000"xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/title | "A High-Throughput Screening Method for the Directed Evolution of Hydroxynitrile Lyase towards Cyanohydrin Synthesis."xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/33146944 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33146944 |
| http://purl.uniprot.org/citations/33146944 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33146944 |
| http://purl.uniprot.org/uniprot/#_O24243-mappedCitation-33146944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33146944 |
| http://purl.uniprot.org/uniprot/O24243 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33146944 |