http://purl.uniprot.org/citations/33166004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/33166004 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectiveTo examine the role of high-fat and high-sugar (HFHS) diet-induced oxidative stress, which is a risk factor for various diseases, in premature ovarian failure (POF).Materials and methodsOvarian granulosa cells (OGCs) were isolated from mice and cultured in medium supplemented with HFHS and poly (lactic-co-glycolic acid) (PLGA)-cross-linked miR-146b-5p nanoparticles (miR-146@PLGA). RNA and protein expression levels were examined using quantitative real-time polymerase chain reaction and Western blotting, respectively. HFHS diet-induced POF model mice were administered miR-146@PLGA.ResultsThe ovarian tissue of mice fed a HFHS diet exhibited the typical pathological characteristics of POF. HFHS supplementation induced oxidative stress injury in the mouse OGCs, activation of the Dab2ip/Ask1/p38-Mapk signalling pathway and phosphorylation of γH2A.X in vitro and in vivo. The results of the luciferase reporter assay revealed that miR-146 specifically downregulated p38-Mapk14 expression. Meanwhile, co-immunoprecipitation and Western blot analyses revealed that HFHS supplementation upregulated nuclear p38-Mapk14 expression and consequently enhanced γH2A.X (Ser139) phosphorylation. The HFHS diet-induced POF mouse model treated with miR-146@PLGA exhibited downregulated p38-Mapk14 expression in the OGCs, mitigated OGC ageing and alleviated the symptoms of POF.ConclusionsThis study demonstrated that HFHS supplementation activates the Dab2ip/Ask1/p38-Mapk signalling pathway and promotes γH2A.X phosphorylation by inhibiting the expression of endogenous miR-146b-5p, which results in OGC ageing and POF development."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.org/dc/terms/identifier | "doi:10.1111/cpr.12954"xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Chen C."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Gong Z."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Lin J."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Liu T."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Wang Z."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Nie X."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/author | "Dou F."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/name | "Cell Prolif"xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/pages | "e12954"xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/title | "MicroRNA-146b-5p overexpression attenuates premature ovarian failure in mice by inhibiting the Dab2ip/Ask1/p38-Mapk pathway and gammaH2A.X phosphorylation."xsd:string |
http://purl.uniprot.org/citations/33166004 | http://purl.uniprot.org/core/volume | "54"xsd:string |
http://purl.uniprot.org/citations/33166004 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33166004 |
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