Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33174523http://www.w3.org/2000/01/rdf-schema#comment"

Background

PTPRG antisense RNA 1 has been well-documented to exert an oncogenic role in diverse neoplasms. However, the precise role of PTPRG antisense RNA 1 in regulating radiosensitivity of nonsmall cell lung cancer cells remains largely elusive.

Methods

Expression levels of PTPRG antisense RNA 1 and miR-200c-3p in nonsmall cell lung cancer tissues and cells were detected by quantitative real-time polymerase chain reaction, while transcription factor 4 expression was examined by immunohistochemistry and Western blot. After nonsmall cell lung cancer cells were exposed to X-ray with different doses in vitro, Cell Counting Kit-8 assay and colony formation assay were conducted to determine the influence of PTPRG antisense RNA 1 on cell viability. Interaction between miR-200c-3p and PTPRG antisense RNA 1 as well as transcription factor 4 was investigated by dual luciferase reporter assay.

Result

In nonsmall cell lung cancer tissues, the expressions of PTPRG antisense RNA 1 and transcription factor 4 were significantly upregulated, whereas the expression of miR-200c-3p was downregulated. It was also proved that PTPRG antisense RNA 1 and 3'-untranslated region of transcription factor 4 can bind to miR-200c-3p. Under X-ray irradiation, overexpressed PTPRG antisense RNA 1 could promote the viability and enhance the radioresistance of nonsmall cell lung cancer cells, and this effect was partially weakened by miR-200c-3p mimics. Transcription factor 4 was identified as a target gene of miR-200c-3p, which could be positively regulated by PTPRG antisense RNA 1.

Conclusion

PTPRG antisense RNA 1 reduces the radiosensitivity of nonsmall cell lung cancer cells via modulating miR-200c-3p/TCF4 axis."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.org/dc/terms/identifier"doi:10.1177/1533033820942615"xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Huang W."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Tang Y."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Zhang C."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Xi Y."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Jiang D."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Ma Q."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Niu R."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/author"Da L."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/name"Technol Cancer Res Treat"xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/pages"1533033820942615"xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/title"Long Noncoding RNA PTPRG Antisense RNA 1 Reduces Radiosensitivity of Nonsmall Cell Lung Cancer Cells Via Regulating MiR-200c-3p/TCF4."xsd:string
http://purl.uniprot.org/citations/33174523http://purl.uniprot.org/core/volume"19"xsd:string
http://purl.uniprot.org/citations/33174523http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33174523
http://purl.uniprot.org/citations/33174523http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33174523
http://purl.uniprot.org/uniprot/#_E9PH57-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
http://purl.uniprot.org/uniprot/#_A0A1B0GW91-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
http://purl.uniprot.org/uniprot/#_A0A1B0GWD5-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
http://purl.uniprot.org/uniprot/#_G0LNT1-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
http://purl.uniprot.org/uniprot/#_G0LNT2-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
http://purl.uniprot.org/uniprot/#_G0LNT3-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
http://purl.uniprot.org/uniprot/#_G0LNT4-mappedCitation-33174523http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33174523
▶