| http://purl.uniprot.org/citations/33229438 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33229438 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33229438 | http://www.w3.org/2000/01/rdf-schema#comment | "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a β-coronavirus, is the causative agent of the COVID-19 pandemic. Like for other coronaviruses, its particles are composed of four structural proteins: spike (S), envelope (E), membrane (M), and nucleoprotein (N) proteins. The involvement of each of these proteins and their interactions are critical for assembly and production of β-coronavirus particles. Here, we sought to characterize the interplay of SARS-CoV-2 structural proteins during the viral assembly process. By combining biochemical and imaging assays in infected versus transfected cells, we show that E and M regulate intracellular trafficking of S as well as its intracellular processing. Indeed, the imaging data reveal that S is relocalized at endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) or Golgi compartments upon coexpression of E or M, as observed in SARS-CoV-2-infected cells, which prevents syncytia formation. We show that a C-terminal retrieval motif in the cytoplasmic tail of S is required for its M-mediated retention in the ERGIC, whereas E induces S retention by modulating the cell secretory pathway. We also highlight that E and M induce a specific maturation of N-glycosylation of S, independently of the regulation of its localization, with a profile that is observed both in infected cells and in purified viral particles. Finally, we show that E, M, and N are required for optimal production of virus-like-particles. Altogether, these results highlight how E and M proteins may influence the properties of S proteins and promote the assembly of SARS-CoV-2 viral particles."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra120.016175"xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra120.016175"xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Zhou B."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Zhou B."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Lavillette D."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Lavillette D."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Cosset F.L."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Cosset F.L."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Mathieu C."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Mathieu C."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Boson B."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Boson B."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Denolly S."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Denolly S."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Legros V."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Legros V."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Siret E."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/author | "Siret E."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/33229438 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |