| http://purl.uniprot.org/citations/33249721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33249721 | http://www.w3.org/2000/01/rdf-schema#comment | "Human serine racemase (hSR) catalyzes the biosynthesis of D-serine, an obligatory co-agonist of the NMDA receptors. It was previously found that the reversible S-nitrosylation of Cys113 reduces hSR activity. Here, we show by site-directed mutagenesis, fluorescence spectroscopy, mass spectrometry, and molecular dynamics that S-nitrosylation stabilizes an open, less-active conformation of the enzyme. The reaction of hSR with either NO or nitroso donors is conformation-dependent and occurs only in the conformation stabilized by the allosteric effector ATP, in which the ε-amino group of Lys114 acts as a base toward the thiol group of Cys113. In the closed conformation stabilized by glycine-an active-site ligand of hSR-the side chain of Lys114 moves away from that of Cys113, while the carboxyl side-chain group of Asp318 moves significantly closer, increasing the thiol pKa and preventing the reaction. We conclude that ATP binding, glycine binding, and S-nitrosylation constitute a three-way regulation mechanism for the tight control of hSR activity. We also show that Cys113 undergoes H2 O2 -mediated oxidation, with loss of enzyme activity, a reaction also dependent on hSR conformation."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.15645"xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Bruno S."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Mozzarelli A."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Marchesani F."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Bettati S."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Campanini B."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Michielon A."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Faggiano S."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Gianquinto E."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Spyrakis F."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/author | "Autiero I."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/name | "FEBS J"xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/pages | "3034-3054"xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/title | "The allosteric interplay between S-nitrosylation and glycine binding controls the activity of human serine racemase."xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://purl.uniprot.org/core/volume | "288"xsd:string |
| http://purl.uniprot.org/citations/33249721 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33249721 |
| http://purl.uniprot.org/citations/33249721 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33249721 |
| http://purl.uniprot.org/uniprot/#_Q8N3F4-mappedCitation-33249721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33249721 |
| http://purl.uniprot.org/uniprot/#_Q3ZK31-mappedCitation-33249721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33249721 |
| http://purl.uniprot.org/uniprot/#_Q53G11-mappedCitation-33249721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33249721 |
| http://purl.uniprot.org/uniprot/#_Q9GZT4-mappedCitation-33249721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33249721 |
| http://purl.uniprot.org/uniprot/Q9GZT4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33249721 |