| http://purl.uniprot.org/citations/33386847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33386847 | http://www.w3.org/2000/01/rdf-schema#comment | "Whole transcriptome analyses have revealed that mammalian genomes are massively transcribed, resulting in the production of huge numbers of transcripts with unknown functions (TUFs). Previous research has categorized most TUFs as noncoding RNAs (ncRNAs) because most previously studied TUFs do not encode open reading frames (ORFs) with biologically significant lengths [>100 amino acids (AAs)]. Recent studies, however, have reported that several transcripts harbouring small ORFs that encode peptides shorter than 100 AAs are translated and play important biological functions. Here, we examined the translational capacity of transcripts annotated as ncRNAs in human cells, and identified several hundreds of ribosome-associated transcripts previously annotated as ncRNAs. Ribosome footprinting and polysome profiling analyses revealed that 61 of them are potentially translatable. Among them, 45 were nonnonsense-mediated mRNA decay targets, suggesting that they are productive mRNAs. We confirmed the translation of one ncRNA, LINC00493, by luciferase reporter assaying and western blotting of a FLAG-tagged LINC00493 peptide. While proteomic analysis revealed that the LINC00493 peptide interacts with many mitochondrial proteins, immunofluorescence assays showed that its peptide is mitochondrially localized. Our findings indicate that some transcripts annotated as ncRNAs encode peptides and that unannotated peptides may perform important roles in cells."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.org/dc/terms/identifier | "doi:10.1093/jb/mvaa143"xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Kawamura T."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Yada T."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Akimitsu N."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Imamachi N."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Taniue K."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Tanu T."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/author | "Yeasmin F."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/name | "J Biochem"xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/pages | "421-434"xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/title | "Identification and analysis of short open reading frames (sORFs) in the initially annotated noncoding RNA LINC00493 from human cells."xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://purl.uniprot.org/core/volume | "169"xsd:string |
| http://purl.uniprot.org/citations/33386847 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33386847 |
| http://purl.uniprot.org/citations/33386847 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33386847 |
| http://purl.uniprot.org/uniprot/#_A0A096LP01-mappedCitation-33386847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33386847 |
| http://purl.uniprot.org/uniprot/A0A096LP01 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33386847 |