| http://purl.uniprot.org/citations/33465374 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33465374 | http://www.w3.org/2000/01/rdf-schema#comment | "Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography-mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in the late stages of retinal development. The mRNA levels of Elovl2 and Elovl4, genes encoding enzymes essential for the synthesis of very-long-chain polyunsaturated fatty acids, increased in developing photoreceptors. Cell sorting based on CD73 expression followed by LC-MS revealed distinct changes in PC and PE levels in CD73-positive rod photoreceptors and CD73-negative retinal cells. Finally, using the NaIO3-induced photoreceptor degeneration model, we identified photoreceptor-specific changes in PC and PE levels from 1 day after NaIO3 administration, before the outer segment of photoreceptors displayed morphological impairment. In conclusion, our findings provide insight into the dynamic changes in PC and PE levels in the developing and adult mouse retina under physiological and pathological conditions. Furthermore, we provide evidence that cell sorting followed by LC-MS is a promising approach for investigating the relevance of lipid homeostasis in the function of different retinal cell types."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jbc.2021.100303"xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Shimizu T."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Watanabe S."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Nagata K."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Sagara H."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Shindou H."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Hamano F."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Kuribayashi H."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Tsuhako A."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/author | "Iwagawa T."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/pages | "100303"xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/title | "Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://purl.uniprot.org/core/volume | "296"xsd:string |
| http://purl.uniprot.org/citations/33465374 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33465374 |
| http://purl.uniprot.org/citations/33465374 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33465374 |
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| http://purl.uniprot.org/uniprot/#_Q543J1-mappedCitation-33465374 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33465374 |
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| http://purl.uniprot.org/uniprot/#_Q9EQC4-mappedCitation-33465374 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33465374 |
| http://purl.uniprot.org/uniprot/#_X2EXH0-mappedCitation-33465374 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33465374 |
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