Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/33485391http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33485391http://www.w3.org/2000/01/rdf-schema#comment"

Background

Herpes Simplex Virus type I (HSV-1) is a large double-stranded DNA virus that enters productive infection in epithelial cells and reorganizes the host nucleus. Cohesin, a major constituent of interphase and mitotic chromosomes comprised of SMC1, SMC3, and SCC1 (Mcd1/Rad21), SCC3 (SA1/SA2), have diverse functions, including sister chromatid cohesion, DNA double-stranded breaks repair, and transcriptional control. Little is known about the role of cohesin in HSV-1 lytic infection.

Methods

We measured the effect on HSV-1 transcription, genome copy number, and viral titer by depleting cohesin components SMC1 or Rad21 using RNAi, followed by immunofluorescence, qPCR, and ChIP experiments to gain insight into cohesin's function in HSV-1 transcription and replication.

Results

Here, we report that cohesion subunits SMC1 and Rad21 are recruited to the lytic HSV-1 replication compartment. The knockdown results in decreased viral transcription, protein expression, and maturation of viral replication compartments. SMC1 and Rad21 knockdown leads to the reduced overall RNA pol II occupancy level but increased RNA pol II ser5 phosphorylation binding on viral genes. Consistent with this, the knockdown increased H3K27me3 modification on these genes.

Conclusions

These results suggest that cohesin facilitates HSV-1 lytic transcription by promoting RNA Pol II transcription activity and preventing chromatin's silencing on the viral genome."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.org/dc/terms/identifier"doi:10.1186/s12985-021-01495-2"xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Chen G."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Cao X."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Li L."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Li X."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Zhou J."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Yang L."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Yu Y."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Lang F."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Wang E."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/author"Fraser N.W."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/name"Virol J"xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/pages"26"xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/title"Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes."xsd:string
http://purl.uniprot.org/citations/33485391http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/33485391http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33485391
http://purl.uniprot.org/citations/33485391http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33485391
http://purl.uniprot.org/uniprot/#_A0A384MR33-mappedCitation-33485391http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33485391
http://purl.uniprot.org/uniprot/#_A8K7A6-mappedCitation-33485391http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33485391
http://purl.uniprot.org/uniprot/#_B3KUT8-mappedCitation-33485391http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33485391
http://purl.uniprot.org/uniprot/#_B7Z709-mappedCitation-33485391http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33485391
▶