Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33550957http://www.w3.org/2000/01/rdf-schema#comment"

Background

Long non-coding RNA FGD5 antisense RNA 1 (FGD5-AS1), identified to be a carcinogenic lncRNA, exhibits a regulatory role in some malignancies including non-small cell lung cancer (NSCLC). The aim of the present research is to decipher the function and underlying mechanism of FGD5-AS1 in progression of NSCLC.

Methods

Expression of FGD5-AS1, miR-493-5p and DEAD-box protein 5 (DDX5) in NSCLC tissues and cells was quantified utilizing qRT-PCR. Cell proliferation was assessed by CCK-8 method. Scratch healing test and Transwell assay were used for assaying cell migration and invasion. Expressions of DDX5 and epithelial-mesenchymal transition (EMT)-related proteins were examined by Western blot. Additionally, targeting relationships between FGD5-AS1 and miR-493-5p, miR-493-5p and DDX5 were verified by dual-luciferase reporter gene assay.

Results

Expression of FGD5-AS1 in NSCLC tissues and cell lines was up-regulated. Expression of FGD5-AS1 was in association with enlarged tumor size and lymph node metastasis of the patients. Knockdown of FGD5-AS1 led to the inhibition of proliferation, migration, invasion and EMT of NSCLC cells. FGD5-AS1 directly targeted miR-493-5p, while DDX5 was the target of miR-493-5p in NSCLC cells. Additionally, FGD5-AS1 could positively regulate the expression of DDX5 via suppressing miR-493-5p.

Conclusion

FGD5-AS1 facilitates the proliferation, migration, invasion and EMT of NSCLC cells by sponging miR-493-5p and up-regulating DDX5."xsd:string
http://purl.uniprot.org/citations/33550957http://purl.org/dc/terms/identifier"doi:10.1177/1533033821990007"xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/author"Bai Y."xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/author"Meng J."xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/author"Cui F."xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/author"Luo P."xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/name"Technol Cancer Res Treat"xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/pages"1533033821990007"xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/title"Silencing of Long Non-Coding RNA FGD5-AS1 Inhibits the Progression of Non-Small Cell Lung Cancer by Regulating the miR-493-5p/DDX5 Axis."xsd:string
http://purl.uniprot.org/citations/33550957http://purl.uniprot.org/core/volume"20"xsd:string
http://purl.uniprot.org/citations/33550957http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33550957
http://purl.uniprot.org/citations/33550957http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33550957
http://purl.uniprot.org/uniprot/#_B4DNG2-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/#_B4DN41-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/#_B5BUE6-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/#_J3KTA4-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/#_P17844-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/#_Q59E92-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/#_Q7Z2V5-mappedCitation-33550957http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/Q7Z2V5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/J3KTA4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/B4DN41http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33550957
http://purl.uniprot.org/uniprot/B4DNG2http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33550957
▶