| http://purl.uniprot.org/citations/33556175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33556175 | http://www.w3.org/2000/01/rdf-schema#comment | "Genome-wide association studies linked expression of the human neutrophil antigen 3b (HNA-3b) epitope on the Slc44a2 protein with a 30% decreased risk of venous thrombosis (VT) in humans. Slc44a2 is a ubiquitous transmembrane protein identified as a receptor for von Willebrand factor (VWF). To explain the link between Slc44a2 and VT, we wanted to determine how Slc44a2 expressing either HNA-3a or HNA-3b on neutrophils could modulate their adhesion and activation on VWF under flow. Transfected HEK293T cells or neutrophils homozygous for the HNA-3a- or HNA-3b-coding allele were purified from healthy donors and perfused in flow chambers coated with VWF at venous shear rates (100 s-1). HNA-3a expression was required for Slc44a2-mediated neutrophil adhesion to VWF at 100 s-1. This adhesion could occur independently of β2 integrin and was enhanced when neutrophils were preactivated with lipopolysaccharide. Moreover, specific shear conditions with high neutrophil concentration could act as a "second hit," inducing the formation of neutrophil extracellular traps. Neutrophil mobilization was also measured by intravital microscopy in venules from SLC44A2-knockout and wild-type mice after histamine-induced endothelial degranulation. Mice lacking Slc44a2 showed a massive reduction in neutrophil recruitment in inflamed mesenteric venules. Our results show that Slc44a2/HNA-3a is important for the adhesion and activation of neutrophils in veins under inflammation and when submitted to specific shears. The fact that neutrophils expressing Slc44a2/HNA-3b have a different response on VWF in the conditions tested could thus explain the association between HNA-3b and a reduced risk for VT in humans."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood.2020008345"xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Thomas G.M."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Robert P."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Alessi M.C."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Morange P.E."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Legendre P."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Lenting P.J."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Tilburg J."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Maracle C.X."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "van Vlijmen B.J.M."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Zirka G."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/author | "Tishkova V."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/pages | "2256-2266"xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/title | "Impaired adhesion of neutrophils expressing Slc44a2/HNA-3b to VWF protects against NETosis under venous shear rates."xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://purl.uniprot.org/core/volume | "137"xsd:string |
| http://purl.uniprot.org/citations/33556175 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33556175 |
| http://purl.uniprot.org/citations/33556175 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33556175 |
| http://purl.uniprot.org/uniprot/#_A0A088QCU6-mappedCitation-33556175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33556175 |
| http://purl.uniprot.org/uniprot/#_A0A024R798-mappedCitation-33556175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33556175 |
| http://purl.uniprot.org/uniprot/#_A0A0S2YRM7-mappedCitation-33556175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33556175 |
| http://purl.uniprot.org/uniprot/#_A0A858XAB0-mappedCitation-33556175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33556175 |