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http://purl.uniprot.org/citations/33565061http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33565061http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To compare the mRNA level of cell proliferation-related genes Twist1, SIRT1, FGF2 and TGF-β3 in placenta mesenchymal stem cells (PA-MSCs), umbilical cord mensenchymals (UC-MSCs) and dental pulp mesenchymal stem cells (DP-MSCs).

Methods

The morphology of various passages of PA-MSCs, UC-MSCs and DP-MSCs were observed by microscopy. Proliferation and promoting ability of the three cell lines were detected with the MTT method. Real-time PCR (RT-PCR) was used to determine the mRNA levels of Twist1, SIRT1, FGF2, TGF-β3.

Results

The morphology of UC-MSCs and DP-MSCs was different from that of PA-MSCs. Proliferation ability and promoting ability of the PA-MSCs was superior to that of UC-MSCs and DP-MSCs. In PA-MSCs, expression level of Twist1 and TGF-β3 was the highest and FGF2 was the lowest. SIRT1 was highly expressed in UC-MSCs. With the cell subcultured, different expression levels of Twist1, SIRT1, FGF2, TGF-β3 was observed in PA-MSCs, UC-MSCs and DP-MSCs.

Conclusion

Up-regulated expression of the Twist1, SIRT1 and TGF-β3 genes can promote proliferation of PA-MSCs, UC-MSCs and DP-MSCs, whilst TGF-β3 may inhibit these. The regulatory effect of Twist1, SIRT1, FGF2 and TGF-β3 genes on PA-MSCs, UC-MSCs and DP-MSCs are different."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.org/dc/terms/identifier"doi:10.3760/cma.j.cn511374-20191217-00641"xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Deng Y."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Huang J."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Sun Z."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Zhang F."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Tan Y."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Zhang R."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Peng H."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Gu X."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/author"Peng K."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/name"Zhonghua Yi Xue Yi Chuan Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/pages"117-122"xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/title"[Expression of Twist1, SIRT1, FGF2 and TGF-beta3 genes and its regulatory effect on the proliferation of placenta, umbilical cord and dental pulp mesenchymal stem cells]."xsd:string
http://purl.uniprot.org/citations/33565061http://purl.uniprot.org/core/volume"38"xsd:string
http://purl.uniprot.org/citations/33565061http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33565061
http://purl.uniprot.org/citations/33565061http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33565061
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http://purl.uniprot.org/uniprot/#_Q15672-mappedCitation-33565061http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33565061
http://purl.uniprot.org/uniprot/#_A8K128-mappedCitation-33565061http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33565061
http://purl.uniprot.org/uniprot/#_B3KVH9-mappedCitation-33565061http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33565061
http://purl.uniprot.org/uniprot/#_A0A9Y1ZCD8-mappedCitation-33565061http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33565061
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