| http://purl.uniprot.org/citations/3357775 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3357775 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3357775 | http://www.w3.org/2000/01/rdf-schema#comment | "A recombinant plasmid has been designed to express the gene encoding a type I methotrexate-resistant dihydrofolate reductase, derived from the bacterial plasmid R483, in DHFR-Chinese hamster ovary cells. Vectors containing the wild type gene, whose coding sequence initiates with a GTG codon, fail to direct the synthesis of detectable levels of protein. Substitution of the GTG codon by an AG codon using in vitro mutagenesis overcomes this block; cells transfected with the modified vector synthesize a functional prokaryotic protein that sustains the growth of these cells in the presence of dihydrofolic acid in the culture media. This property is consistent with the inability of the type I enzyme to reduce folate to dihydrofolate, and enabled the development of a selection strategy whereby prokaryotic and mammalian DHFRs genes could be used sequentially as independently selectable markers."xsd:string |
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| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/author | "Levinson A.D."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/author | "Levinson A.D."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/author | "Walter M."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/author | "Walter M."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/author | "Simonsen C.S."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/author | "Simonsen C.S."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/pages | "2235-2246"xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/pages | "2235-2246"xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/title | "Expression of the plasmid-encoded type I dihydrofolate reductase gene in cultured mammalian cells: a novel selectable marker."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/title | "Expression of the plasmid-encoded type I dihydrofolate reductase gene in cultured mammalian cells: a novel selectable marker."xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/3357775 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3357775 |
| http://purl.uniprot.org/citations/3357775 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3357775 |
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| http://purl.uniprot.org/citations/3357775 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3357775 |