| http://purl.uniprot.org/citations/33581333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33581333 | http://www.w3.org/2000/01/rdf-schema#comment | "Inside mitochondria reside semi-autonomous genomes, called mtDNA. mtDNA is multi-copy per cell and mtDNA copy number can vary from hundreds to thousands of copies per cell. The variability of mtDNA copy number between tissues, combined with the lack of variability of copy number within a tissue, suggest a homeostatic copy number regulation mechanism. Mutations in the gene encoding the Caenorhabditis elegans hydroxylase, CLK-1, result in elevated mtDNA. CLK-1's canonical role in ubiquinone biosynthesis results in clk-1 mutants lacking ubiquinone. Importantly, clk-1 mutants also exhibit slowed biological timing phenotypes (pharyngeal pumping, defecation, development) and an activated stress response (UPRmt). These biological timing and stress phenotypes have been attributed to ubiquinone deficiency; however, it is unknown whether the mtDNA phenotype is also due to ubiquinone deficiency. To test this, in animals carrying the uncharacterized clk-1 (ok1247) mutant allele, we supplemented with an exogenous ubiquinone precursor 2-4-dihydroxybenzoate (DHB), which has previously been shown to restore ubiquinone biosynthesis. We measured phenotypes as a function of DHB across a log-scale range. Unlike the biological timing and stress phenotypes, the elevated mtDNA phenotype was not rescued. Since CLK-1's canonical role is in ubiquinone biosynthesis and DHB does not rescue mtDNA copy number, we infer CLK-1 has an additional function in homeostatic mtDNA copy number regulation."xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mito.2021.02.001"xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/author | "Patel M.R."xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/author | "Kirby C.S."xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/name | "Mitochondrion"xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/pages | "38-48"xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/title | "Elevated mitochondrial DNA copy number found in ubiquinone-deficient clk-1 mutants is not rescued by ubiquinone precursor 2-4-dihydroxybenzoate."xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://purl.uniprot.org/core/volume | "58"xsd:string |
| http://purl.uniprot.org/citations/33581333 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33581333 |
| http://purl.uniprot.org/citations/33581333 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33581333 |
| http://purl.uniprot.org/uniprot/#_P48376-mappedCitation-33581333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33581333 |
| http://purl.uniprot.org/uniprot/P48376 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33581333 |