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http://purl.uniprot.org/citations/33590535http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33590535http://www.w3.org/2000/01/rdf-schema#comment"Sphingolipids are ubiquitous structural components of eukaryotic cell membranes which are vital for maintaining the integrity of cells. Alkaline ceramidase is a key enzyme in sphingolipid biosynthesis pathway; however, little is known about the role of the enzyme in the male reproductive system of Drosophila melanogaster. To investigate the impact of alkaline ceramidase (Dacer) on male Drosophila, we got Dacer deficiency mutants (MUs) and found they displayed apparent defects in the testis's phenotype. To profile the molecular changes associated with this abnormal phenotype, we performed de novo transcriptome analyses of the MU and wildtype (WT) testes; and revealed 1239 upregulated genes and 1102 downregulated genes. Then, six upregulated DEGs (papilin [Ppn], croquemort [Crq], terribly reduced optic lobes [Trol], Laminin, Wunen-2, collagen type IV alpha 1 [Cg25C]) and three downregulated DEGs (mucin related 18B [Mur18B], rhomboid-7 [Rho-7], CG3168) were confirmed through quantitative real-time polymerase chain reaction in WT and MU samples. The differentially expressed genes were mainly associated with catalytic activity, oxidoreductase activity and transmembrane transporter activity, which significantly contributed to extracellular matrix-receptor interaction, fatty acids biosynthesis as well as glycine, serine, and threonine metabolism. The results highlight the importance of Dacer in the reproductive system of D. melanogaster and provide valuable resources to dig out the specific biological functions of Dacer in insect reproduction."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.org/dc/terms/identifier"doi:10.1002/arch.21765"xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Mao C."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Zhang C."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Zhou W."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Wang N."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Ali S.A."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Zhang C.'"xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Zhang M.J."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Zhu Z.R."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Shi X.X."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/author"Quais M.K."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/name"Arch Insect Biochem Physiol"xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/pages"e21765"xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/title"Transcriptional changes revealed genes and pathways involved in the deficient testis caused by the inhibition of alkaline ceramidase (Dacer) in Drosophila melanogaster."xsd:string
http://purl.uniprot.org/citations/33590535http://purl.uniprot.org/core/volume"106"xsd:string
http://purl.uniprot.org/citations/33590535http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33590535
http://purl.uniprot.org/citations/33590535http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33590535
http://purl.uniprot.org/uniprot/#_A0A023GRW4-mappedCitation-33590535http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33590535
http://purl.uniprot.org/uniprot/#_A0A0B4LEJ6-mappedCitation-33590535http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33590535
http://purl.uniprot.org/uniprot/#_A1Z7U3-mappedCitation-33590535http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33590535
http://purl.uniprot.org/uniprot/#_A1Z8R8-mappedCitation-33590535http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33590535
http://purl.uniprot.org/uniprot/#_A0A6H2EG25-mappedCitation-33590535http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33590535