| http://purl.uniprot.org/citations/33596023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33596023 | http://www.w3.org/2000/01/rdf-schema#comment | "Our previous research has shown that monocytic leukemia-associated antigen-34 (MLAA-34) was a novel antiapoptotic molecule with unique expression in acute monocytic leukemia (M5), making it an ideal target for T-cell-based immunotherapy. Here, we sought to identify HLA-A*0201-restricted cytotoxic T-lymphocyte (CTL) epitope of MLAA-34 by reverse immunology. In all, 10 HLA-A*0201 restricted epitopes of MLAA-34 were predicted by bioinformatics. MLAA-34324-332, MLAA-34293-301, and MLAA-34236-244 showed the strongest HLA-A*0201-binding affinity. The percentages of HLA-A*0201-MLAA-34236-244 tetramer+ CD8+ T cells in MLAA-34236-244-induced CTLs were raised apparently. Enzyme-linked immunospot showed that MLAA-34236-244 and MLAA-34324-332-specific CTLs produced a higher amount of interferon-γ. MLAA-34236-244-induced CTLs presented a stronger cytotoxic effect on THP-1 cells (HLA-A*0201+MLAA-34+) at various effector to target ratios. MLAA-34236-244 peptide vaccine could inhibit the tumor growth and improve mean survival time of leukemia-bearing human peripheral blood lymphocyte reconstituting severe combined immunodeficient mice. Mice immunized with MLAA-34236-244 vaccine had increased percentages of MLAA-34236-244 tetramer+ CD8+ T cells in the spleen after each round of immunization. High-purity CD8+ and CD4+ T cells were sorted by Dynabeads as effector cells. The killing activity of CD8+ T cells was higher than that of CD4+ T cells. CTLs derived from the MLAA-34 peptide vaccine group were significantly higher than other therapeutic groups and showed specific cytotoxicity to THP-1 cells. Increased interferon-γ and interleukin (IL)-2 and decreased IL-10 and IL-4 were seen in the MLAA-34236-244 peptide vaccine group. MLAA-34236-244 peptide (ILDRHNFAI) is an effective HLA-A*0201-restricted CTL epitope and that it may serve as a promising strategy in designing antigen-specific immunotherapy against MLAA-34-positive acute monocytic leukemia."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.org/dc/terms/identifier | "doi:10.1097/cji.0000000000000350"xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/author | "Zhang W."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/author | "Bai J."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/author | "He A."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/name | "J Immunother"xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/pages | "141-150"xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/title | "Identification of HLA-A*0201-restricted CTL Epitopes for MLAA-34-specific Immunotherapy for Acute Monocytic Leukemia."xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://purl.uniprot.org/core/volume | "44"xsd:string |
| http://purl.uniprot.org/citations/33596023 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33596023 |
| http://purl.uniprot.org/citations/33596023 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33596023 |
| http://purl.uniprot.org/uniprot/#_Q9H9S4-mappedCitation-33596023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33596023 |
| http://purl.uniprot.org/uniprot/#_Q86WE6-mappedCitation-33596023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33596023 |
| http://purl.uniprot.org/uniprot/Q9H9S4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33596023 |
| http://purl.uniprot.org/uniprot/Q86WE6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33596023 |