Results

RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/33639023http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33639023http://www.w3.org/2000/01/rdf-schema#comment"

Problem

The miRNAs show placenta-specific expression patterns, which alter during pregnancy-related complications. In present study, the role of miR-27b-5p during forskolin-mediated BeWo cells fusion has been investigated.

Method of study

The fusion of BeWo cells in response to forskolin treatment (25 µM) was studied by desmoplakin I+II staining. Expression profile of miR-27b-5p by qRT-PCR and its targets HSD3β1 and WNT2B by qRT-PCR and in Western blot were studied. The effect of overexpression of miR-27b-5p and silencing of HSD3β1 & WNT2B by siRNA on forskolin-mediated BeWo cells fusion and secretion of hCG and progesterone by ELISA was investigated.

Results

Time-dependent down-regulation in the expression of miR-27b-5p in forskolin-treated BeWo cells has been confirmed by qRT-PCR. Overexpression of miR-27b-5p significantly inhibits forskolin-mediated BeWo cells fusion as well as hCG & progesterone secretion. HSD3β1 and WNT2B were identified as targets of miR-27b-5p and are up-regulated in forskolin-treated BeWo cells. Overexpression of miR-27b-5p in BeWo cells downregulates their expression. Further, luciferase reporter assay revealed that miR-27b-5p directly target expression of both HSD3β1 and WNT2B. Silencing of both HSD3β1 and WNT2B leads to a significant reduction in forskolin-mediated BeWo cells fusion with concomitant decrease in the secretion of progesterone or/and hCG. Decrease in forskolin-mediated cells fusion observed in miR-27b-5p mimic transfected BeWo cells could be rescued by the overexpression of both HSD3β1 and WNT2B.

Conclusion

These observations suggest that reduced miR-27b-5p in forskolin-treated BeWo cells leads to increased secretion of progesterone and hCG due to loss of repressional control on HSD3β1 and WNT2B."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.org/dc/terms/identifier"doi:10.1111/aji.13409"xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Gupta S.K."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Mishra R."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Malik A."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Verma S."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Panda A.K."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Chaudhary P."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/author"Malhotra S.S."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/name"Am J Reprod Immunol"xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/pages"e13409"xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/title"miR-27b-5p inhibits BeWo cells fusion by regulating WNT2B and enzyme involved in progesterone synthesis."xsd:string
http://purl.uniprot.org/citations/33639023http://purl.uniprot.org/core/volume"86"xsd:string
http://purl.uniprot.org/citations/33639023http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33639023
http://purl.uniprot.org/citations/33639023http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33639023
http://purl.uniprot.org/uniprot/#_P14060-mappedCitation-33639023http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33639023
http://purl.uniprot.org/uniprot/#_Q5TEH8-mappedCitation-33639023http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33639023
http://purl.uniprot.org/uniprot/#_Q93097-mappedCitation-33639023http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33639023
http://purl.uniprot.org/uniprot/Q93097http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33639023
http://purl.uniprot.org/uniprot/P14060http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33639023
http://purl.uniprot.org/uniprot/Q5TEH8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33639023